牦牛病毒性腹泻病病毒E2基因的克隆及序列分析  

Cloning and Sequence Analysis of the E2 Gene of Bovine Viral Diarrhea Virus Strain Yak

作　　者：胡炳峰[1] 刘亚刚[1] 王文伯[1] 杨晓农[1] 于吉峰[2] 冯英阳[1] 王盼盼[1] 

机构地区：[1]西南民族大学生命科学与技术学院,成都610041 [2]四川省畜牧科学研究院,成都610066

出　　处：《中国畜牧兽医》2010年第2期93-96,共4页China Animal Husbandry & Veterinary Medicine

基　　金：四川省科技厅攻关项目(04ZY029-006-04);国家民委项目(07XN03);西南民族大学研究生创新型科研项目资助

摘　　要：根据GenBank中已发表的多株牛病毒性腹泻病毒的E2基因序列比较分析设计了3对引物,应用RT-PCR方法首次扩增出牦牛病毒性腹泻病毒的E2基因区637 bp的片段序列,经pMD18-T载体连接后克隆、测序,并与已发表的NADL、Osloss、Oregon C24V等毒株E2基因序列进行系统发育进化树比对分析,结果表明,牦牛病毒性腹泻病毒的E2基因无插入或缺失,系统发育进化分析表明,与其他毒株核苷酸同源性较低,最高仅为68.8%,推导氨基酸序列为68.4%。较低的同源性表明牦牛病毒性腹泻病毒存在较大的基因突变或者可能还具有独立的衍化来源。According to the E2 genes of bovine viral diarrhea virus（BVDV） which were obtained from GenBank,the target fragment of about 640 bp of isolate Yak was amplified by reverse transcription polymerase chain reaction（RT-PCR）,cloned into pMD18-T vector and sequenced.The positive recombinant clone was identified by restriction enzyme digestion.Sequence comparison and phylogenetic analyses showed that isolate Yak fall into the BVDV genotype 1 and were distant from other subgroups.There is no insertion or variation of E2 gene.It had the highest sequence identity（68.8%） with the Australia Braidwood strain.The low sequence identity with other BVDV-1 strains indicate that strain Yak had an independent evolutional pathway.

关 键 词：牦牛病毒性腹泻病毒 E2基因 克隆 序列分析 

分 类 号：Q78[生物学—分子生物学]