石歧杂鸡催乳素基因外显子2的SNP检测与产蛋就巢性状的关联分析  

SNP Detection of Exon 2 of PRL Gene and its Associations with Egg-laying and Broodiness Traits in Shiqiza Chicken

作　　者：曹顶国[1,2] 李福伟[1,2] 雷秋霞[1,2] 韩海霞[1,2] 周艳[1,2] 李桂明[1,2] 黄保华[1,2] 逯岩[1,2] 

机构地区：[1]山东省农业科学院家禽研究所 [2]山东省家禽育种工程技术中心,济南250023

出　　处：《中国畜牧兽医》2010年第2期104-107,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家行业科技计划(NYHYZX07-039);山东省良种工程项目(2007LZ014);山东省农业科学院创新基金

摘　　要：利用PCR-SSCP技术对石歧杂鸡的催乳素(prolactin,PRL)基因外显子2进行SNP检测和测序分析,并对该基因与石歧杂鸡产蛋、就巢性状的相关性进行了研究。结果表明,PRL基因外显子2存在多态性位点,该位点由于在3838位点发生C→T的碱基突变,产生了AA、AB和BB3种基因型,并导致氨基酸的改变,由亮氨酸变为苯丙氨酸。基因型AA和等位基因A的频率最高;BB基因型个体首次就巢时间显著晚于AA和AB基因型(P<0.01);BB基因型的就巢次数显著多于AA和AB基因型(P<0.05);3种基因型与产蛋性状相关性不显著。Exon 2 of prolactin gene were detected by PCR-SSCP and the DNA fragments were sequenced.The correlation analysis between the polymorphism of the exon 2 and egg-laying and broodiness traits were carried out respectively.The results indicated that there were two polymorphic sites in exon 2.A C/T mutation at the position of the 3838 bp was found,which resulted in 3 genotypes（AA,AB and BB）.Furthermore,an amino acid changed from Leucine to Phenylalanine.The frequencies of genotype AA and allele A were the highest.The least square analysis showed that the first broodiness day of BB type chickens was later than AA and AB type chickens（P〈0.01）,the times of broody of BB chickens were more than AA and AB type（P〈0.05）.But there was no significant difference in egg production among the 3 genotypes.

关 键 词：PRL基因 PCR-SSCP 石歧杂鸡 就巢性状 

分 类 号：Q754[生物学—分子生物学]