丙酮在细菌ArgRS纯化过程中的作用  

Acetone acts on the Purification of Bacterial ArgRS

作　　者：严世荣[1] 朱明磊[1] 熊秀敏[1] 

机构地区：[1]郧阳医学院

出　　处：《郧阳医学院学报》1998年第2期80-81,共2页Journal of Yunyang Medical College

摘　　要：目的：纯化细菌的精氨酸－ｔＲＮＡ合成酶。方法：培养大肠杆菌、酵母菌、嗜热脂肪芽胞杆菌，分别采用超声波破菌和丙酮破菌、层析、测活力峰。结果：酵母菌、大肠杆菌用不同和破菌方法纯化结果完全相同，丙酮破菌法显著提高嗜热脂肪芽胞杆菌ＡｒｇＲＳ的纯化。结论：丙酮在破菌过程中去掉了嗜热脂肪芽胞杆菌体内的大量脂类化合物，提高了纯化效率。Objective:Purification of bacterial Argining tRNA synthetase(ArgRS).Methods:The purification of ArgRS for Escherichia Coli.Yeast and Bacillus stearothermophilus(Bst) includ breaking bacterium in Ultrasonic and acetone.Chromtography and assaing activity.Results:Breaking Bst in acetone Heighten the purification efficiency of Bst's ArgRS.Conclusion:Because acetone can remove a lot of lipid chemical compound of Bst,this method can shorten the time and simplify the process of enzyme purification

关 键 词：丙酮 破菌 精氨酰 TRNA合成酶 酶纯化 细菌 

分 类 号：Q550.3[生物学—生物化学] Q936