红叶芥低温胁迫下苯丙氨酸解氨酶活性及其基因的克隆表达  

Cloning and Expression of PAL Gene and PAL Activity Assay in Red-Leaf Mustard(Brassica juncea var.garrhiza Tsen et Lee) under Low Temperature Stress

作　　者：孙梓健[1] 汤青林[1] 宋明[1] 任雪松[1] 

机构地区：[1]西南大学园艺园林学院,重庆市蔬菜学重点实验室,重庆400716

出　　处：《西南大学学报（自然科学版）》2010年第2期90-94,共5页Journal of Southwest University(Natural Science Edition)

基　　金：重庆市自然科学基金资助项目(2009BB1307)

摘　　要：用8℃低温处理红叶芥四叶期植株,在低温处理的52 h内分别取样6次,取样后分别提取叶片总RNA,再利用RT-PCR克隆得到红叶芥PAL基因开放阅读框内1 908 bp片段,核苷酸序列分析显示,红叶芥PAL基因与芜青PAL基因的同源性达到99%.半定量RT-PCR结果显示,在8℃低温胁迫下,红叶芥PAL基因表达量呈现先降低后升高的趋势,在处理18 h后达到最低点,随后迅速升高,这与PAL活性变化一致.The 4-leaf seedlings of red-leaf mustard（Brassica juncea var.garrhiza Tsen et Lee） were exposed to low temperature（8 ℃） for 52 h,during which period leaf samples were taken 6 times for total RNA extraction.The open reading frame fragment that was generated by RT-PCR using specific primers was 1 908 bp.The cDNA from the leaves of red-leaf mustard was used as the template.Nucleotide sequence analysis showed that red-leaf mustard PAL gene shared 99% identity to that of Brassica napus.In response to the low-temperature stress in this experiment,the expression of PAL first dropped and then increased rapidly,being the lowest 18 h after the start of the treatment,a similar trend to that of PAL activity.

关 键 词：低温胁迫 红叶芥 苯丙氨酸解氨酶(PAL)活性 PAL基因克隆表达 花青素 

分 类 号：Q949.748.3[生物学—植物学]