地塞米松诱导人小梁细胞bcl-2基因的表达  

Expression of bcl-2 Gene in Human Trabecular Cells Induced by Dexamethasone

作　　者：卓业鸿[1] 葛坚[1] 林明楷[1] 郑健樑[1] 李艳艳[1] 

机构地区：[1]中山医科大学中山眼科中心,广州510060

出　　处：《眼科学报》1998年第4期187-189,共3页Eye Science

基　　金：国家自然科学基金(编号39770789;39800163);广东省科委基金(编号980112)

摘　　要：目的:探讨地塞米松对小梁细胞bcl-2基因表达的影响。方法:应用组织块培养的方法建立人小梁细胞培养。取第三代小梁细胞培养于载玻片上,含10^(-7)mol/L地塞米松的培养液作用6小时、12小时、24小时后,应用LSAB观察bcl-2基因表达的情况。结果:地塞米松作用6小时后可见bcl-2蛋白阳性细胞,且随时间的延长,阳性细胞有所增多。结论:地塞米松可诱导小梁细胞bcl-2基因表达,可能在激素性青光眼发病中起着一定的作用。眼科学报1998;14:187～189。Purpose:To investigate the influence of dexamethasone on expression of bcl-2 gene in human trabecular cells. Methods: The trabecular specimens from human eyes were cultured in vitro and the cultured cells of the third generation on slides were immersed in culture solution containing 10-7M dexamethasone for 6 hours, 12 hours and 24 hours respectively. The expression of bcl-2 gene was studied with LSAB. Results: The positive cells of the bcl-2 protein could be identified after dexamethasone effected for 6 hours and the positive cell increased with time going on. Conclusion :The expression of bcl-2 gene can be induced by dexamethasone, it may be related with glaucomatic pathogenesis. Eye Science 1998 ; 14 ; 187 - 189.

关 键 词：地塞米松 小梁细胞 BCL-2基因 青光眼 病理学 

分 类 号：R775.902[医药卫生—眼科]