柔红霉素脂质体的制备及兔房水中柔红霉素及其脂质体浓度的测定  

Preparation of Liposome Encapsulated Daunorbicine and Determination of Daunorubicine of Aqueous Humor in Rabbit Eyes

作　　者：吴明星[1] 利华明[1] 李绍珍[1] 潘苏华[1] 

机构地区：[1]中山医科大学中山眼科中心,广州510060

出　　处：《眼科学报》1998年第4期232-235,共4页Eye Science

摘　　要：目的:探讨药物柔红霉素(daunorubicine,DNR)及其脂质体在房水中维持的浓度及时间。方法:20只兔眼于晶体囊外摘除术中分别注入0.1ml之1mg/ml DNR及其脂质体(LDNR),术后12、24、48小时和1周时间采集房水,应用高效液相色谱法测定房水中DNR的浓度。结果:术后12、24、48小时注入DNR和LDNR眼均可测出DNR,且LDNR眼浓度明显高于DNR眼,术后1周LDNR眼仍可测出DNR,但注入DNR眼无法测出。结论:应用高效液相色谱法可测定兔房水中的DNR浓度,且LDNR眼浓度明显高于DNR眼,有明显的缓释延时效应。眼科学报1998;14:232～235。Purpose: To investigate the method of determination the concentration of daunoru-bicine(DNR)and liposome encapsulated daunorubicine( LDNR)of aqueous humor in rabbit eyes. Methods: After extracapsular lens extraction, 0. 1ml of 1mg/ml DNR and LDNR were injected into the anterior chamber. At the 12,24,48 hours and 1 week after operation , the aqueous humor were aspirated to determine the concentration of DNR by high performance liquid chromatography(HPLC). Results : At the 12,24,48 hours after operation,DNR were determined in all eyes,but at one week after operation, the DNR could only be deteded in LDNR group. The concentration of DNR in LDNR group were higher than DNR group. Eye Science 1998; 14:232 - 235.

关 键 词：柔红霉素 脂质体 房水 后囊膜混浊 

分 类 号：R776.105[医药卫生—眼科] R978.115[医药卫生—临床医学]