葡萄酒中槲皮素和山奈酚的高效液相色谱化学发光测定法  

Simultaneous Determination of Quercetin and Kaempferol in Wine by High Performance Liquid Chromatography with Chemiluminescence Detection

作　　者：高智席[1] 刘焱[1] 曾启华[1] 唐国风[1] 周光明[2] 蒋开燕 

机构地区：[1]遵义师范学院化学系,贵州遵义563002 [2]西南大学化学化工学院,重庆北碚400715 [3]重庆市万州区国本中学,重庆万州404100

出　　处：《环境与健康杂志》2010年第2期145-146,共2页Journal of Environment and Health

基　　金：贵州省教育厅自然科学青年项目(黔教科2007072);贵州省遵义市科技局项目([2008]26号)

摘　　要：目的建立葡萄酒中槲皮素和山奈酚的高效液相色谱化学发光测定法。方法基于在NaOH碱性介质中铁氰化钾可以直接氧化槲皮素和山奈酚产生化学发光原理,用高效液相色谱分离柱后化学发光法测定葡萄酒中槲皮素和山奈酚含量。结果槲皮素和山奈酚浓度分别在2.0×10-6～1.6×10-2g/m(lr=0.9990)和3.2×10-6～2.0×10-4g/m(lr=0.9998)范围内与化学发光强度呈良好的线性关系,检出限(S/N=3)分别为1.4×10-9g/ml和3.4×10-7g/ml,回收率分别为99.31%和99.06%。RSD分别为2.07%,1.29%。结论该法具有简便、快速、准确等优点,适合葡萄酒中槲皮素和山奈酚的测定。Objective To establish a new method for the determination of quercetin and kaempferol in wine by high performance liquid chromatography （HPLC） with chemiluminescence. Methods The procedure was based on the chemiluminescent enhancement by quercetin and kaempferol of the K3Fe（CN）6 system in an alkaline medium. The HPLC with post-column chemiluminescence was established for the determination of quercetin and kaempferol in wine. Results The standard curves were linear in the concentration ranges of 2.0×10^6-1.6×10^-2 g/ml （r=-0.999 0） for quercetin and 3.2×10^-6-2.0×10^-4 g/ml （r=0.999 8）for kaempfero respectively.The limits of detection were 1.4 ×10^-9 g/ml for quercetin and 3.4 ×10^-7 g/ml （S/N =3）for kaempfero respectively. The rates of recovery were 99.31% for quercetin and 99.06% for kaempferol. Conclusion The method is simple, rapid, accurate and applicable to the determination of quercetin and kaempferol in wine.

关 键 词：色谱法 液相 化学发光 葡萄酒 槲皮素 山奈酚 

分 类 号：O657.7[理学—分析化学]