大豆农艺性状的QTL分析  

QTL Analysis of Agronomic Traits in Soybean

作　　者：吕祝章[1] 杨建华[1] 李玉环[1] 常汝镇[2] 邱丽娟[2] 

机构地区：[1]日照职业技术学院,山东日照276826 [2]中国农业科学院作物研究所国家农作物基因资源与遗传改良重大科学工程农业部作物种质资源与生物技术重点开放实验室,北京100081

出　　处：《安徽农业科学》2010年第6期2838-2841,共4页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金资助项目(30490250)

摘　　要：[目的]分析大豆农艺性状的QTL,为探讨大豆的遗传机制及进行遗传育种提供参考。[方法]应用复合区间作图法对蛋白质含量、脂肪含量、产量、百粒重、生育期等5个数量性状进行QTL定位和遗传效应分析。[结果]控制蛋白质含量、脂肪含量、产量、百粒重、生育期性状的4、4、1、2、5个共16个QTL位点,遗传贡献率在7.4%～33.7%。其中,遗传贡献率较大的主效QTL有分别位于I连锁群上Satt562-Sat_219、Sat_219-Satt496、Sat_219-Satt496区间的3个控制蛋白质含量的QTL位点,其遗传贡献率分别为29.15%、33.70%和31.67%,且均为来自母本合丰25的加效基因,还有位于O连锁群上Satt477-Satt331、Satt331-Satt153区间的2个控制生育期QTL位点,其遗传贡献率分别为24.69%和24.96%,也是来自母本合丰25的加效基因。另外,6个分别距M连锁群Satt175(蛋白质)、A1连锁群Satt684(油分)、F连锁群Satt348(油分)、J连锁群Sat_412(油分)、C1连锁群Sat_416(百粒重)、C1连锁群Sat_416(生育期)标记仅有0.01 cm的QTL位点。[结论]定位了影响蛋白质含量、油分含量、产量、百粒重和生育期等5个重要农艺性状的QTL位点。[Objective] The aim was to analyze QTL of agronomic traits in soybean and provide reference for a discussion on soybean genetic mechanism and genetic breeding.[Method] The composite interval mapping method was used for QTL location and genetic effects analysis on five quantitative traits,including protein content,fat content,yield,100-seed weight and growing period.[Result] The control of these traits 4,4,1,2,5,a total of 16 QTL loci was detected.The genetic contribution rate was in 7.4%-33.7%,among which,a large main-effect QTL of the genetic contribution rate were located in linkage group I Satt562-Sat_219,Sat_219-Satt496,Sat_219-Satt496 interval of the three control protein content QTL sites,their genetic contribution rates were 29.15%,33.7 % and 31.67% respectively,all from the female parent Hefeng 25 plus minor gene;still in O linkage group Satt477-Satt331,Satt331-Satt153 interval of two control growing period QTL loci,their genetic contribution rates were up to 24.69% and 24.96%,also from the female parent Hefeng 25 plus minor gene.In addition,six QTL sites from M linkage group Satt175（protein）,A1 linkage group Satt684（oil）,F linkage group Satt348（oil）,J linkage group Sat_412（oil）,C1 linkage group Sat_416（100-seed weight）,C1 linkage group Sat_416（growing period） marks only 0.01 cM were detected.[Conclusion] QTL sites which have effects on the five important agronomic traits in soybean were located.

关 键 词：大豆 复合区间作图 农艺性状 QTL 

分 类 号：S565.1[农业科学—作物学]