利用丁酸合成PHA高效菌株的筛选及摇瓶发酵特性研究  

Isolation of a PHA Producing Strain with Butyric Acid as the Carbon Source and Its Shaking-Flask Fermentation Character

作　　者：陈志强[1,2] 李云蓓[2] 温沁雪[2] 

机构地区：[1]城市水资源开发利用(北方)国家工程研究中心,哈尔滨150090 [2]哈尔滨工业大学城市水资源与水环境国家重点实验室,哈尔滨150090

出　　处：《环境科学》2010年第3期828-832,共5页Environmental Science

基　　金：国家自然科学基金项目(50708025);中国博士后科学基金项目(20080430921);哈尔滨市留学回国人才研究专项资金项目(2007RFLXS013);城市水资源与水环境国家重点实验室开放课题项目(08UWQA06)

摘　　要：从活性污泥中分离到1株能够利用丁酸合成聚羟基脂肪酸酯(PHA)的细菌WD-3,对该菌株积累PHA的发酵特性进行了研究,包括PHA产量随碳氮比、pH、发酵时间的变化,并考察了整个发酵过程中碳源、氮源、生物量、pH值和PHA产量之间的关系.结果表明,当C/N为35,pH为7.0时,WD-3的PHA合成能力最高.在最佳培养条件下,细胞生长达到稳定期时,该菌PHA产量高达3.01 g.L-1,占细胞干重的45.4%,且PHV所占比例达到三分之一.经16S rRNA基因测序分析,初步确定该菌属于γ-变形菌亚纲中的肠杆菌属Enterobacter.A bacterial strain,WD-3,with high polyhydroxyalkanoates（PHA） yield was isolated from activated sludge.Its fermentation characters including the effects of C∶N ratio,pH,fermentation time on the PHA production were also primary studied. The results showed that the optimal C/N ratio and pH were 35 and 7.0,respectively.PHA accumulation increased rapidly during the stable growth stage.The maximum PHA yield was 3.01 g·L^-1,the strain accumulated PHA up to 45.4% of its cellular dry weight,and the proportion of PHV occupied one-third of the PHA production.Analysis of its 16S rRNA sequence showed that strain WD-3 belongs to Enterobacter.

关 键 词：聚羟基脂肪酸酯(PHA) 丁酸 菌株 碳氮比 发酵时间 

分 类 号：X172[环境科学与工程—环境科学]