恒场电泳检测DNA双链断裂及其应用  

DETECTING DNA DOUBLE STRAND BREAKS AFTER INONIZING IRRADIATION BY CONSTANT FILED GEL ELECTROPHORESIS

作　　者：李雨[1] 郑秀龙[1] 罗成基[1] 蔡建明[1] 孟祥顺 吴玮 高建国[1] 

机构地区：[1]第二军医大学放射医学研究所,第三军医大学防原教研室

出　　处：《辐射研究与辐射工艺学报》1998年第4期234-237,共4页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金

摘　　要：应用普通恒场琼脂糖凝胶电泳检测受γ射线照射后小鼠胸腺细胞ＤＮＡ双链断裂（ｄｓｂ）。在一定条件下，该法可以获得与脉冲凝胶电泳相似的结果。用此法观察了离体照射小鼠胸腺细胞ＤＮＡｄｓｂ修复作用，发现该法可以检测到ＤＮＡ“梯状”带，可做为一种研究细胞凋亡的新方法。It was observed that constant field gel electrophoresis can detect DNA double breaks(dsb) by irradiation. The dsb degree of mice thymocyte by 0～50 Gy (irradiation and the dsb repair in two hours after 30Gy irradiation was studied, the “DNA ladder” was also found using the method.

关 键 词：恒场 电泳 Γ射线照射 DNA双链断裂 DSB 

分 类 号：R818.74[医药卫生—放射医学] Q345.1[医药卫生—临床医学]