神经病理性痛大鼠脊髓背角星形胶质细胞NF—κB活性的变化  

Change in activity of NF-κB in astrocytes in spinal dorsal horn in a rat model of neuropathic pain

作　　者：孙涛[1] 左玲[1] 张丽[1] 傅志俭[1] 宋文阁[1] 贾明睿[1] 王公明[1] 王波[1] 魏广福[1] 

机构地区：[1]山东大学附属省立医院疼痛科,济南市250021

出　　处：《中华麻醉学杂志》2010年第1期50-52,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（30801072）;山东省自然科学基金（Q2007C14）

摘　　要：目的观察神经病理性痛大鼠脊髓背角星形胶质细胞NF-κB活性的变化，以探讨脊髓星形胶质细胞调控神经病理性痛时胞内可能的信号转导通路机制。方法雄性sD大鼠16只，月龄2—3月，体重220—280g，随机分为2组（n=8）：假手术组（S组）和神经病理性痛组（CCI组）。CCI组采用慢性压迫性损伤法制备大鼠慢性神经病理性痛模型，s组仅暴露坐骨神经。分别于术前1d和术后7d测定机械痛阈和热痛阈，术后第7天测定痛阈后处死大鼠，取脊髓，记录腰段脊髓背角星形胶质细胞核内NF-κBp65的免疫反应阳性细胞数。结果与术前1d比较，CCI组大鼠术后7d机械痛阈和热痛阈降低（P〈0．05）。与S组比较，CCI组大鼠术后7d机械痛阈和热痛阈降低，术侧脊髓背角星形胶质细胞NF-κBp65免疫阳性细胞数增多（P〈0．05）。结论脊髓背角星形胶质细胞参与大鼠神经病理性痛的调控，其机制可能与NF-κB信号转导通路有关。Objective To investigate the change in NF-κB activity in astrocytes in spinal dorsal horn in a rat model of neuropathic pain and the underlying mechanism. Methods Sixteen male SD rats aged 2-3 months weighing 220-280 g were randomly divided into 2 groups （ n = 8 each） ： sham operation group （group S） and CCI group. Neuropathic pain was induced by chronic constrictive injury （CCI）. Right sciatic nerve was exposed and 4 loose ligatures were placed on the sciatic nerve at 1 mm intervals with 4-0 chromic catgut. In group S the right sciatic nerve was exposed but not ligated. The paw withdrawal threshold （PWT） to yon Frey filament stimulation and paw withdrawal latency （PWL） to radiant heat stimulation were measured at 1 d before （baseline） and 7 d after operation. The animals were then killed and the lumbar segment of the spinal cord （L4-6） was removed. The expression of NF-κB in the astrocytes in spinal dorsal horn was determined by immuno-histochemistry. Results PWT and PWL to mechanical and thermal stimuli were significantly decreased after operation as compared with the baseline before operation in group CCI. The number of NF-κBp65 immunoreaction positive cells in the spinal dorsal horn on the operated side was significantly larger in group CCI than in group S. Conclusion NF-κB signal transduction pathway in the astrocytes in the spinal dorsal horn may be involved in neuropathic pain.

关 键 词：NF-κB 神经痛 脊髓 星形细胞 

分 类 号：R741[医药卫生—神经病学与精神病学]