加巴喷丁对神经病理性痛大鼠背根神经节神经元高电压激活钙电流的影响  

Effects of gabapentin on high voltage active calcium currents in injured dorsal root ganglion neurons in a rat model of neuropathic pain

作　　者：陈晓东[1] 朱敏敏[2] 安珊珊[3] 王强[3] 陆亮[3] 孙芹 肖杭[3] 徐建国[2] 段满林[2] 

机构地区：[1]江苏省麻醉学重点实验室,徐州医学院221002 [2]南京军区南京总医院麻醉科 [3]南京医科大学现代毒理学教育部重点实验室 [4]不详

出　　处：《中华麻醉学杂志》2010年第1期56-59,共4页Chinese Journal of Anesthesiology

摘　　要：目的评价加巴喷丁对神经病理性痛大鼠背根神经节神经元高电压激活钙电流的影响。方法雄性SD大鼠，周龄4—6周，采用结扎k脊神经的方法建立神经病理性痛模型。于术后14d时采用酶消化法急性分离损伤侧L背根神经节神经元，采用全细胞膜片钳技术记录神经元高电压激活钙电流，记录加巴喷丁0．1、1、10、100、300μmol／L（G1-5组）作用下的钙电流，计算电流抑制率；并绘制100p．mol／L加巴喷丁作用下钙电流．电压曲线、钙通道激活曲线和稳态失活曲线。结果与给药前比较，G1-5组给药后钙电流均降低（P〈0.05）。给予100μmol／L加巴喷丁后钙电流．电压曲线左移。与给药前比较，给予100μmol／L加巴喷丁后钙通道激活曲线的半数激活电压和稳态失活曲线的半数失活电压降低（P〈0．05），激活曲线向超级化方向移动3．47mv，稳态失活曲线向超极化方向移动9．32mV。结论加巴喷丁可抑制神经病理性痛大鼠背根神经节神经元高电压激活钙电流，可能与其抑制钙通道的失活特性有关。Objective To investigate the effect of gabapentin on high voltage active calcium currents in the injured dorsal root ganglion （DRG） neurons in a rat model of neuropathic pain. Methods Pathogen-flee male SD rats aged 4-6 weeks were used in this study. The animals were anesthetized with intraperitoneal pentobarbital sodium 50 mg/kg. L5 spinal nerve was ligated between DRG and sciatic nerve and cut distal to the ligature. The animals were decapitated on the 14th postoperative day. L3 DRG was isolated and the neurons in the ganglion were enzymatically dissociated. The high voltage active calcium current was recorded using whole-cell patch-clamp technique.Results Gabapentin inhibited the peak calcium current in the injured DEG neurons. Peak calcium current was decreased by gabapentin 100μmol/L and both activation and steady-state inactivation curve shifted to more hyperpolarized potentials. Conclusion Gabapentin can inhibit high voltage active in the injured DRG neurons in a rat model of neuropathic pain. The alteration in the inactivation of the electrophysiologlcal properties may be involved in the mechanism.

关 键 词：环己酸类 钙通道 神经痛 神经节 脊 神经元 

分 类 号：R74[医药卫生—神经病学与精神病学]