PAN-蛋白质络合物的极谱吸附波及其应用  

A polargraphic absorption wave of PAN-proteins complex and its application

作　　者：郑石英[1] 罗登柏[2] 

机构地区：[1]福建农林大学生命科学学院,福州350002 [2]中南民族大学化学与材料科学学院,武汉430074

出　　处：《分析试验室》2010年第3期44-46,共3页Chinese Journal of Analysis Laboratory

基　　金：湖北省自然科学基金(2005ABA001);福建农林大学校青年基金(08B11)项目资助

摘　　要：在0.05 mol/L HAc溶液中,PAN-牛血清白蛋白/人血清白蛋白络合物在-0.58 V(vs.SCE)处产生一灵敏吸附还原峰,峰电位较之游离PAN还原峰负约0.28 V,峰电流与牛血清白蛋白、人血清白蛋白浓度在0.1～12 mg/L,0.1～11 mg/L范围内呈线性关系;检测限均为0.05 mg/L。运用该法测定了人血清样品蛋白质含量。A sensitive reduction wave of PAN-proteins at-0.58 V（vs.SCE）has been observed by using single sweep polarography in 0.05 mol/L acetic acid and 7.5×10-6 mol/L PAN solution.The peak potential is 0.28 V more negative than that of PAN.The second order derivative peak height is linearly proportional to the concentration of BSA from 0.1 to 12 mg/L and to the concentration of HSA from 0.1 to 11 mg/L.The detection limit is 0.05 mg/L both for BSA and for HSA.The method has been used for the determination of total protein in human serum samples.The result is satisfactory.

关 键 词：单扫描极谱法 1-(2-吡啶偶氮)-2-萘酚(PAN) 牛血清白蛋白(BSA) 人血清白蛋白(HSA)络合物 

分 类 号：O657.1[理学—分析化学]