大鼠再生肝8种细胞的一碳代谢相关基因转录谱研究  

Expression Profiles of One Carbon Metabolism-Associated Genes in 8 Liver Cell Types during Rat Liver Regeneration

作　　者：张春艳[1,2] 于亚男[1] 宗慧[1] 李静雯[1] 徐存拴 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]河南省-科技部共建细胞分化调控国家重点实验室培育基地,河南新乡453007

出　　处：《河南师范大学学报（自然科学版）》2010年第1期181-184,共4页Journal of Henan Normal University(Natural Science Edition)

基　　金：国家973项目前期研究专项资助(2006CB708506)

摘　　要：为了解大鼠肝再生中8种肝脏细胞的一碳代谢相关基因转录谱及其预示的代谢活动,分离大鼠再生肝的8种细胞,检测它们的一碳代谢相关基因表达变化,分析其表达模式及预示的生理活动.结果表明,肝星形细胞的甲硫氨酸转化为S-腺苷甲硫氨酸活动在肝再生启动阶段增强.胆管上皮细胞的S-腺苷甲硫氨酸转化为S-腺苷高半胱氨酸、N5-甲基四氢叶酸将甲基转移给甲硫氨酸活动在进展阶段增强.肝细胞、陷窝细胞、树突状细胞的聚谷氨酸水解、四氢叶酸与N5-甲酰基四氢叶酸相互转化在肝再生3个阶段增强.结论:大鼠肝再生与一碳代谢相关.To explore the transcription profiles of one carbon metabolism-related genes in 8 liver cell types during liver regeneration and the associated metabolism activities indicated by them,the cells are sorted out,their gene expression changes are detected,and their expression patterns and the predicted physiological activities are analyzed. The results suggest that synthesis activities of DNA methyltransferases 3A and 5-methyltetrahy-drofolate-homocysteine methyltransferase become more active at progressing phase of liver regeneration; the synthesis of methylenetetrahy drofolate dehydrogenase in hepatocyte,biliary epithelial cell,pit cell and dendritic cell,and that of folate hydrolase 1 in hepatocyte,sinusoidal endothelial cell,pit cell and dendritic cell are enhanced at three phase of liver regeneration. Conclusion：one carbon metabolism is associated with liver regeneration.

关 键 词：大鼠肝再生 RAT GENOME 230 2.0芯片 基因转录谱 一碳代谢 

分 类 号：Q418[生物学—生理学]