子宫内膜癌组织APE、HIF-1α和VEGF表达及意义  

EXPRESSION AND CLINICAL SIGNIFICANCE OF APE,HIF-1α,AND VEGF IN ENDOMETRIAL CARCINOMA

作　　者：李发红[1] 纪新强[1] 吴景丽[1] 

机构地区：[1]青岛大学医学院附属医院妇科,山东青岛266003

出　　处：《齐鲁医学杂志》2010年第2期101-103,106,共4页Medical Journal of Qilu

摘　　要：目的了解无嘌呤无嘧啶核酸内切酶（APE）、低氧诱导因子-1α（HIF-1α）和血管内皮生长因子（VEGF）在子宫内膜癌中的表达及其意义，并探讨APE与HIF-1α、VEGF蛋白表达之间的关系。方法应用免疫组织化学方法检测APE、HIF-1α和VEGF在44例子宫内膜癌、22例子宫内膜不典型增生及18例正常子宫内膜组织中的表达情况。结果APE、HIF-1α和VEGF在正常子宫内膜、子宫内膜不典型增生及子宫内膜癌组织中表达的阳性率比较，差异均有显著性（χ^2=8．371～18．589，P〈0．05）。APE在各种子宫内膜组织细胞内的定位有所不同，正常子宫内膜组织APE表达于细胞核内，子宫内膜不典型增生组织中6例（42．86％）细胞质中有APE表达，子宫内膜癌组织中24例（70.59％）细胞质有APE表达，APE在3种组织细胞内定位差异有显著性（χ^2=12．701，P〈0．05）。而且细胞质中有APE表达的子宫内膜癌较无表达者VEGF阳性率显著增加（χ^2=3．973，P〈0．05）。APE蛋白在子宫内膜癌组织细胞质中的阳性表达率与肿瘤的分化程度及病理分期有关（χ^2=11．059、9．969，P〈0．01、0．05）。子宫内膜癌组织APE及HIF-1α的表达均与VEGF的表达有相关性（r=0．626、0．469，P〈0．01），且APE与HIF-1α的表达也有相关性（r=0．612，P〈O．01）。结论子宫内膜癌组织中APE、HIF-I~和VEGF蛋白参与子宫内膜癌的发生发展，APE蛋白与HIF—1α可能通过上调VEGF蛋白的表达，促进肿瘤的血管生成而促进子宫内膜癌的发生。Objective To study the expression of apurinic endonuclease （APE）, hypoxia-indueible factor-1 alpha （HIF- 1α）, and vascular endothelial growth factor （VEGF） in endometrial eareinoma （EC） and investigate the relationship between APE, and HIF-1α and VEGF protein expression. Methods Immunohistoehemistry teehnique was used to detect the expression of APE, HIF-1α, and VEGF protein in 44 cases of EC, 22 of atypical hyperplasia and 18 of normal endometrium. Results The expression rates of APE, HIF-1α, and VEGF in normal endometrium, atypieal hyperplasia and EC were significantly different among the three groups （χ^2= 8. 371-18. 589, P〈0.05）. The location of APE inside endometrial cells was different in the three different endometrim tissues. APE was expressed in the nucleus of normal endometrial cells. Cytoplasm location of APE was found in six eases （42. 86%） of atypieal hyperplasia and in 24 （70.59%） of EC, the difference of location was significant among the three different endometrium tissues （χ^2 = 12. 701, P〈0.05）. The expression of the VEGF in EC with positive cytoplasm APE expression was significantly higher than in EC without eytoplasm APE expression （χ^2 = 3. 973,P〈0.05）. The APE expression rate in EC was associated with the differentiation and pathological staging of the tumor （χ^2= 11. 059,9. 969;P〈0. 01,0. 05）. The expression of APE and HIF-1α in EC was positively correlated with VEGF expression （r=0. 626,0. 469;P〈0.01） and the expression of APE was also positively correlated with HIF-1α （r=0. 612,P〈0.01）. Conclusion APE, HIF-1α, and VEGF are involved in the earcinogenesis and development of EC. It is probably that APE and HIF-1α upregulate the expression of VEGF, promote angiogenesis and result in the occurrence of the cancer.

关 键 词：子宫内膜肿瘤 DNA-(无嘌呤或无嘧啶位点)裂合酶 低氧诱导因子-1Α 血管内皮生长因子类 免疫组织化学 

分 类 号：R737.33[医药卫生—肿瘤]