灵芝多糖的结构特征分析  

Analysis of Structural Characteristics of Polysaccharide from Ganoderma Lucidum

作　　者：何晋浙[1] 邵平[1] 孟祥河[1] 孙培龙[1] 

机构地区：[1]浙江工业大学生物与环境工程学院食品系,杭州310032

出　　处：《分析化学》2010年第3期372-376,共5页Chinese Journal of Analytical Chemistry

基　　金：国家高技术研究发展计划863计划(No.2007AA10Z337);浙江省科技厅项目(No.2008C32030)资助

摘　　要：采用沸水回流法从灵芝子实体中提取多糖,经Sevage法除蛋白,乙醇沉淀,离心、透析、膜分离,浓缩、冻干后得灵芝多糖。经HIO4氧化、Smith降解及甲基化反应,并利用多糖及刚果红混合液在碱性溶液中的波长的红移变化,通过UV-VIS,IR,GC,GC-MS,NMR对灵芝水提多糖的结构特征及三螺旋体结构进行分析研究。结果表明:灵芝多糖含有三螺旋体构型,GC-MS分析灵芝多糖的主要单糖组分为葡萄糖,还有少量的半乳糖、甘露糖、木糖和艾杜糖,IR及1HNMR分析多糖为β-构型,HIO4氧化、Smith降解和甲基化分析表明:多糖主要为(1→3)糖苷键连接构型,并伴有少量的1→6位支链键连接的结构,灵芝多糖是由D-葡萄糖单元通过β-(1→3)糖苷键连接葡聚多糖,其主要构型特征为(1→3)β-D-线性连接的骨架结构。Polysaccharide was extracted by boiling water reflux method from the fruiting body of Ganoderma lucidum. Additionally,the purified polysaccharide was obtained by removing protein with Sevage way,ethanol precipitation,centrifugation,run water dialysis,membrane separation,concentration and frozen-drying. The structural characteristics,chain conformation and triple-helix conformation of ganoderma lucidum polysaccharide （ GLP） were distinguished by Smith degradation,methylation analysis,and the wavelength change of the red shift of the mixture of polysaccharide and Congo red in alkaline solution,as well as IR,GC-MS,NMR, and visible spectrometry. The results indicated that GLP was a linear （ 1→3） β-D-Glcp main chain linkage. Its monosaccharide component was predominantly composed of D-Glc,and small amount of galactose,mannose,xylose and idose,residues of branches terminated with substituted at 1→6 by a small number of singleunit β-D-Glcp side-chains,it′s also observed that the （ 1→3）-linked β-D-glucan contained a triple-helical conformation,which was composed of a repeating unit with a structure as below：

关 键 词：灵芝多糖 结构特征 Β-葡聚糖 三螺旋体 

分 类 号：R284.1[医药卫生—中药学]