表面等离子体共振生物传感器连续检测莱克多巴胺  

Continuous Detection Method for Ractopamine with Surface Plasmon Resonance Biosensor

作　　者：李辉[1] 蔡浩原[1] 陈兴[1] 孙建海[1] 张璐璐[1] 崔大付[1] 

机构地区：[1]中国科学院电子学研究所传感技术国家重点实验室,北京100191

出　　处：《分析化学》2010年第3期381-384,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(Nos.60701019;60976088);中国科学院重大科研装备研制项目(No.YZ200807);国家高技术研究发展计划(863计划)(No.2007AA10Z431)资助

摘　　要：利用表面等离子体共振生物传感器对莱克多巴胺抗体与固定在芯片表面的莱克多巴胺衍生物的相互作用进行了分析,解离常数为2.56×10-6s-1。根据一定范围内相对响应值和时间近似呈线性关系的动力学特性,建立了连续检测的方法,从而简化了实验步骤,有利于提高芯片的使用寿命。检测莱克多巴胺采用抑制法,将莱克多巴胺衍生物固定在芯片的表面,莱克多巴胺抗体与样品混合后流过芯片的表面,所得相对响应值与样品中莱克多巴胺的浓度成反比。单个样品的检测时间设定为15min,对应的检出限小于4μg/L。The interaction between the anti-Ractopamine （ Rac） monoclonal antibody and the Rac derivation immobilized on the sensor chip surface was studied with surface plasmon resonance （ SPR） biosensor. A continuous detection method based on the linear response during the association phase was developed. The sensor chip surface was regenerated after several tests performed continuously,thus the detection step was simplified and the life span of the chip was prospected to be prolonged. The detection was performed as an inhibitive immunoassay. The mixture of anti-Rac monoclonal antibody and the sample flowed over the surface with Rac derivation was immobilized. The relative response was in inversely proportion to the concentration of Rac. The detection limit was less than 4 μg/L with a detection time of 15 min.

关 键 词：表面等离子体共振 生物传感器 动力学 连续检测 莱克多巴胺 

分 类 号：R155[医药卫生—营养与食品卫生学] R318.6[医药卫生—公共卫生与预防医学]