鳗鲡肌肉冻干粉中呋喃唑酮代谢物3-氨基-2-噁唑烷酮残留标准物质的研制  

Preparation and Certification of Reference Materials for 3-Amino-2-oxazolidinone Residue in Eel Muscle Lyophilisates

作　　者：杨方[1] 杨守深[2] 卢声宇[1] 刘正才[1] 余孔捷[1] 李耀平[1] 储晓刚[3] 

机构地区：[1]福建出入境检验检疫局,福州350001 [2]福建农林大学动物科学学院,福州350003 [3]中国检验检疫科学研究院,北京100123

出　　处：《分析化学》2010年第3期397-400,共4页Chinese Journal of Analytical Chemistry

基　　金：“十一·五”国家科技支撑计划项目(No.2006BAK02A08);福建省科技攻关重点项目(No.2006Y0002)资助

摘　　要：建立了鳗鲡肌肉冻干粉中3-氨基-2-噁唑烷酮(AOZ)残留分析标准物质的研制和定值方法。以约0.16mg/L呋喃唑酮对鳗鲡进行药浴给药,使呋喃唑酮在鱼体中代谢,从而使鳗鲡肌肉中含有一定浓度的呋喃唑酮代谢物AOZ。经冻干、真空包装及辐照等处理后获得1批400个独立包装的肌肉冻干粉样本。经过均匀性与稳定性检验,由11家实验室采用同位素稀释液相色谱-串联质谱法进行协同定值,并进行了不确定度评估,已被国家质检总局批准确为国家二级标准物质。A methodology for preparing and certifying the reference material of 3-amino-2-oxazolidinone （ AOZ） in eel muscle lyophilisates was presented. Furazolidone was accessed to eel by dipping fish in pond with furazolidone solution at a dosage of ca 0. 16 mg/L. With the metabolism of furazolidone in eel,the muscles contain a certain concentration of AOZ as furazolidone metabolite was obtained. Lyophilization of the muscles was performed in one batch and 400 bags of samples were obtained by the procedure of homogenation, cryodesiccation and irradiation. The homogeneity and stability of the sample was examined. The value of the chemical constituent of the sample was certified through the collaborative analysis program participated by 11 laboratories using isotope dilution liquid chromatography-tandem mass spectrometry,and the uncertainty assessment was performed. The reference materials have been approved as certified reference materials by AQSIQ,China （ State General Administration of the People′s Republic of China for Quality Supervision and Inspection and Quarantine） in 2009 after one year of trial period. The serial numbers is GBW（ E） 100180.

关 键 词：标准物质 3-氨基-2-噁唑烷酮 鳗鲡肌肉 残留 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]