I型鸭肝炎病毒RT-PCR检测方法的建立  

Establishment of RT-PCR method for detecting Duck Hepatitis Virus I

作　　者：魏雪涛[1] 张晓勇 李银[1] 刘宇卓[1] 张敬峰[1] 

机构地区：[1]江苏省农业科学院兽医研究所农业部动物疫病诊断与免疫重点开放实验室国家兽用生物制品工程技术研究中心,江苏南京210014 [2]浙江省嘉兴市出入境检验检疫局,浙江嘉兴314001

出　　处：《浙江农业学报》2010年第1期10-13,共4页Acta Agriculturae Zhejiangensis

基　　金：江苏省农业自主创新资金项目(cx(09)117)

摘　　要：根据GenBank发表的DHVI的基因序列,设计了1对针对DHVI保守区的特异性引物。通过对反应条件的优化,建立了鸭病毒性肝炎病毒快速鉴别诊断的RT-PCR方法,扩增片段大小为632bp。试验表明,RT-PCR法具有很强的特异性,且该方法的敏感性明显高于常规的检测方法。对鸭病毒性肝炎的病料检测证明,该方法能有效鉴别DHV,特异性良好。According to the conserved gene sequence of Duck Hepatitis Virus I（DHV I） in GenBank,one pair of the specific primers were designed. A rapid diagnostic technique of RT-PCR was established by optimizing the reaction condition. The DNA segments amplified were 632 bp. The experiments had proved that RT-PCR possessed a high specificity. And the sensitivity test results indicated that the RT-PCR was more sensitive than the conventional method. Detection of field samples from viral diseased ducks also confirmed that the method of RT-PCR could effectively identify the infection of DHV.

关 键 词：I型鸭肝炎病毒 RT-PCR 诊断 

分 类 号：S852[农业科学—基础兽医学]