SDS,Cu^(2+)污染对蟾蜍蝌蚪的急性毒性及肝脏Na^+-K^+-AT-Pase活性的影响  

Acute toxicity of SDS,Cu^(2+) pollution and its effect on Na^+-K^+-ATPase activity in the liver of Bufo gargarizans

作　　者：金叶飞[1] 祝尧荣[1] 韩丽[1] 

机构地区：[1]绍兴文理学院生命科学学院,浙江绍兴312000

出　　处：《浙江农业学报》2010年第1期81-86,共6页Acta Agriculturae Zhejiangensis

基　　金：绍兴市科技局计划项目(2008A34005)

摘　　要：以中华大蟾蜍(Bufo gargarizans)蝌蚪为试验材料,采用联合指数相加法,研究了阴离子表面活性剂十二烷基硫酸钠(SDS)、重金属Cu2+对蟾蜍蝌蚪的急性毒性和联合毒性效应;并在急性毒性试验的基础上,研究了亚急性条件下SDS,Cu2+及其复合污染对蝌蚪肝脏Na+-K+-ATPase活性的影响。试验结果表明,SDS对蝌蚪的48,72,96h的半致死浓度(LC50)分别为27.38,26.45,25.57mg/L;Cu2+对蝌蚪的48,72,96h的半致死浓度(LC50)分别为0.475,0.323,0.285mg/L。SDS,Cu2+对蝌蚪的联合毒性作用48h内表现为毒性增强的协同效应,96h后表现为拮抗作用。蝌蚪在亚急性浓度的SDS,Cu2+条件下暴露4d后,SDS,Cu2+及复合污染均对蝌蚪肝脏的Na+-K+-ATPase活性产生明显影响,SDS,Cu2+复合污染在Na+-K+-ATPase水平上表现为一定的协同作用。To investigate the effects of single or combined sodium dodecyl sulfate （SDS） and Cu^2＋ pollution in Bufo gargarizans,the acute toxicity and joint toxicity tests of SDS,Cu^2＋ and Na^＋-K^＋-ATPase activity in liver under the subacute toxicities of single or combined SDS and Cu^2＋ were measured by using exposure experiment. The results showed that the LC50 of SDS for 48,72 and 96 h to Bufo gargarizans were 27.38,26.45 and 25.57 mg·L^-1,respectively,and the LC50 of Cu^2＋ for 48,72 and 96 h were 0.475,0.323 and 0.285 mg·L^-1,respectively. The toxicity of Cu^2＋ was higher than that of SDS. In addition,it also indicated that the joint toxicity of Cu^2＋ and SDS was synergistic at 48 h but antagonistic at 96 h. The results also indicated that single or combined treatments with Cu^2＋ and SDS could affect the Na^＋-K^＋-ATPase activity in liver of Bufo gargarizans,and the joint effect of the combined pollution on the Na^＋-K^＋-ATPase activity was synergistic.

关 键 词：铜 十二烷基硫酸钠 中华大蟾蜍蝌蚪 急性毒性 联合毒性 NA+-K+-ATP酶 

分 类 号：X171[环境科学与工程—环境科学]