P16蛋白失表达对肝外胆管癌细胞增殖的影响  

THE INFLUENCE ON CARCINOMA OF THE EXTRAHEPATIC BILE DUCT OF P16 ABNORMAL EXPRESSION

作　　者：蔡圣强[1] 陈澍周[2] 

机构地区：[1]扬州大学医学院附属靖江医院普外科,江苏22500 [2]南通大学医学院附属医院普外科

出　　处：《交通医学》2010年第1期7-9,共3页Medical Journal of Communications

摘　　要：目的:探讨P16蛋白失表达对肝外胆管癌细胞增殖的影响,分析其与肝外胆管癌患者临床特征和预后的关系。方法:应用S-P法检测35例肝外胆管癌和12例慢性胆管炎标本中P16和Ki-67的表达。结果:P16蛋白在肝外胆管癌中阳性表达率25.7%,显著低于慢性胆管炎阳性表达率66.7%(P=0.0446)。肝外胆管癌中P16蛋白阳性表达与淋巴结转移呈负相关(P=0.015)。肝外胆管癌和慢性胆管炎Ki-67标记指数(Ki-67LI)分别为34.6±19.5和16.3±12.8,差异有统计学意义(P<0.05)。Ki-67LI在临床Ⅰ、Ⅱ期和Ⅲ、Ⅳ,淋巴结是否转移差异有统计学意义(P均<0.05)。结论:P16蛋白失表达影响肝外胆管癌细胞增生,与肝外胆管癌发生、发展密切相关。Objective：To determine the influence of abnomal expression of P16 protein in cholangiocarcinoma speciments,and to analyses the predictive value to patients with cholangiocarcinoma.Methods：Formalin fixed,paraffin embedded specimens from 35 patients with cholangiocarcinoma were immunostained with P16 and Ki-67 antibody by stretaridine/peroxidase immunochemical assays and 12 cases of cholangitis were collected as contrast.Statistical methods were used to detect the significance.Results：The positive staining rate of P16 protein in cholangitis specimens was significantly higher than that in cholangiocarcinoma specimens.It was higher when lymph node metastasis had occurred.The Ki-67LI was 16.3±12.8 in cholangitis specimens and was 34.6±19.5 in cholangiocarcinoma specimens.The difference was stastical in the Ki-67LI for tumors with clinical Ⅲ and Ⅳstages,and with lymph node metastasis significantly exceeding that without these.Conclusion：The abnomal expression of P16 protein affects proliferation of cholangiocarcinoma cell,and has correlation with the occurrence of cholangiocarcinoma.

关 键 词：肝外胆管癌 P16 KI-67 免疫组织化学 

分 类 号：R735.8[医药卫生—肿瘤]