促黄体生成激素酶促化学发光免疫分析方法的建立  

Chemiluminescence Immunoassay for Luteinizing Hormone

作　　者：刘佳宁[1] 刘一兵[1] 贾娟娟[1] 许文革[1] 张雪峰[1] 韩世泉[1] 

机构地区：[1]中国原子能科学研究院同位素研究所,北京102413

出　　处：《同位素》2010年第1期28-33,共6页Journal of Isotopes

摘　　要：采用双抗体夹心一步检测法和鲁米诺-过氧化氢发光体系建立了血清促黄体生成激素（LH）的酶促化学发光免疫分析方法。结果显示，本方法测量范围为1．5-200IU／L，灵敏度为0．08IU／L，批内变异系数〈9％，批间变异系数〈11％，回收率为96．3％～112．1％，稀释实验测定值与稀释度呈线性相关，相关系数r=0．995。与进口酶促化学发光免疫分析试剂盒测定的临床值进行比较，线性相关方程为y=0．967x＋0．0689，相关系数r=0．975，相关性良好。方法学鉴定结果符合免疫分析的基本要求。The chemiluminescence immunoassay（CLIA） for serum luteinizing hormone was established by using the reaction of luminol with hydrogen peroxide. The measurement range of this method was 1.5 to 200 IU/L, the sensitivity was 0.08 IU/L, the recovery rate was 96.3% to 112.1%, the coefficient correlation of dilution,was 0. 995, and the intra- and inter-assay coefficient of variability were 4.09%-8.36% and 5. 14%-10.23%, respectively. Compared with Beckman CLIA system, the coefficient correlation was 0. 975.

关 键 词：促黄体生成激素 酶促化学发光免疫分析 分析方法 

分 类 号：R446.61[医药卫生—诊断学]