透明质酸高产菌株选育研究  

Study on Screening and Mutation of High Hyaluronic Acid-Producing Strain

作　　者：叶倩文[1] 卢陆洋[1] 李新松[1] 

机构地区：[1]东南大学化学化工学院生物材料和药物释放实验室,江苏南京211189

出　　处：《化学与生物工程》2010年第3期51-54,共4页Chemistry & Bioengineering

摘　　要：从新鲜水牛鼻粘膜分离筛选得到15株菌株,经生化反应鉴定其中5株为透明质酸(HA)产生菌。对产量最高的菌株B发酵液精提物进行红外吸收光谱(IR)、核磁共振(13CNMR)、紫外吸收光谱(UV)分析,证明其发酵液精提物确为HA。菌株B经超声波/紫外线复合诱变处理后,HA产量由0.793 g.L-1提高到1.46 g.L-1。15 Strains were isolated from fresh buffalo nasal mucosa through screening. Five of the obtained strains were confirmed to be hyaluronic acid（HA）-producing strains by biochemical reaction. The strain B with the highest HA yield was cultured and its fermentation extract was characterized by IR, 13 CNMR and UV spec- tra. The results indicated that the strain B could produce HA. When strain B was treated by physical mutation of ultrasonic wave combined with UV-irradiation, the HA yield increased from 0. 793 g ·L^-1 to 1.46 g·L^-1.

关 键 词：透明质酸高产菌株 筛选 鉴定 物理诱变 

分 类 号：TQ921.7[轻工技术与工程—发酵工程]