血芝液体培养及条件优化的初步研究  

The Preliminary Research of Liquid Culture of Xuezhi and Optimization of Culture Conditions

作　　者：马海昇[1] 李海珍[1] 姚志伟[1] 

机构地区：[1]武汉生物工程学院生物工程系,湖北武汉430415

出　　处：《化学与生物工程》2010年第3期70-72,共3页Chemistry & Bioengineering

摘　　要：研究了血芝液体培养的条件。结果显示，在碳源为葡萄糖、氮源为牛肉粉、温度为25～27℃、pH值为5的优化培养条件下，血芝菌丝生长良好。同时，通过正交实验确定血芝液体培养的最佳碳、氮源组合[g·（100mL）^-1]为：葡萄糖2．0、玉米粉1．0、黄豆粉1．5、酵母粉0．1，其中，黄豆粉对血芝生长影响最为显著。The liquid culture conditions of Xuezhi was explored in this study. The results showed that the mycelium growed well under the optimum culture conditions as follows：glucose was carbon source, beef flour was nitrogen source, the temperature was 25-27℃, and the pH value was 5. Meanwhile, the best combination for growth of Xuezhi was determined by orthogonal experiment,glucose 2.0 g ·（100mL）^-1 , corn flour 1.0 g ·（100mL）^-1 , soybean powder 1.5 g ·（100mL）^-1 , yeast extract 0. 1 g·（100mL）^-1.

关 键 词：血芝 液体培养 培养条件 正交实验 

分 类 号：TQ929.2[轻工技术与工程—发酵工程] S567.31[农业科学—中草药栽培]