铜绿微囊藻胞内毒素(Microcystin-LR)提取条件的优化实验  

Optimization for the Extraction of Microcystin-LR from Microcystis aeruginosa Cells

作　　者：王祥荣[1] 任晶[1] 卢虹虹[1] 樊正球[1] 

机构地区：[1]复旦大学环境科学与工程系,上海200433

出　　处：《复旦学报（自然科学版）》2010年第1期99-103,共5页Journal of Fudan University：Natural Science

基　　金：上海市教委科创基金资助项目(08ZZ03);教育部博士点专项科研基金资助项目(20060246024);上海市自然科学基金资助项目(08ZR1401300);复旦-汾湖科技产业化基金资助项目

摘　　要：结合HPLC定量分析了藻毒素提取条件对提取纯培养铜绿微囊藻(Microcystis aeruginosa)胞内毒素效率的影响,优化并建立了胞内藻毒素Microcystin-LR(MCLR)提取流程:藻液离心后中加入w(甲醇)=75%萃取剂,冻融和超声破碎后离心收集上清液并进行固相萃取,分别以w(甲醇)=10%淋洗,以w(甲醇)=80%+w(TFA)=0.05%洗脱SPE柱,最后收集到较纯的微囊藻毒素MCLR.The extraction procedure of mierocystin-LR （MCLR） from intracellular Microcystis aeruginosa 905 was optimized. Alga slurry was collected through centrifugation, and then w （methanol） = 75% was added as extraction solvent. The mixed liquid was treated with freeze-thawing followed by ultrasonic for 0. 5 h, and the supernatant was collected for solid phase extraction （SPE）. The optimized SPE conditions were also studied： C18 cartridge was eluted with w （methanol） =10% and then washed with w （methanol） =80% and w （TFA） =0. 05%. As a result, the MCLR with better purity was obtained.

关 键 词：铜绿微囊藻 MCLR HPLC 优化 

分 类 号：X131.2[环境科学与工程—环境科学]