RNA干扰IGF-1受体和EGF受体表达对鼻咽癌裸鼠移植瘤细胞周期的影响  

Effect of Dual Silencing of IGF-1 and EGF Receptors on PCNA Labeling Index and Mitotic Count of Human Nasopharyngeal Carcinoma Xenografts in Nude Mice and Cell Cycle of Nasopharyngeal Carcinoma Cells

作　　者：卢硕[1] 孟小萍[2] 周绪红[3] 袁玉林[1] 彭涛[3] 李俊[3] 叶林峰[3] 

机构地区：[1]武汉大学基础医学院人体解剖学教研室,湖北武汉430071 [2]武汉大学基础医学院法医学教研室 [3]武汉大学中南医院耳鼻咽喉科

出　　处：《咸宁学院学报（医学版）》2010年第1期15-20,共6页Journal of Xianning Univarsity(medical Sciences)

摘　　要：目的探讨RNA干扰IGF-1受体和EGF受体表达对鼻咽癌裸鼠移植瘤的增殖状态和鼻咽癌CNE2细胞的细胞周期趋势的影响及其作用机制。方法应用免疫组织化学S-P方法和形态计量技术对鼻咽癌裸鼠移植瘤(实验组和对照组)组织切片进行PCNA染色、HE染色以及流式细胞仪检测鼻咽癌CNE2细胞(实验组和对照组)在细胞各期的细胞比例和增殖指数。结果实验组鼻咽癌皮下移植瘤PCNA标记指数为27.19±10.20,核分裂像计数为18.80±7.47;质粒对照组和正常对照组鼻咽癌皮下移植瘤PCNA标记指数别为36.47±9.03和37.07±8.63,核分裂像计数分别为37.53±5.25和38.03±6.15,同一组的两指标显著正相关(P<0.01)。实验组鼻咽癌CNE2细胞处于G0/G1期、S期和G2/M期的细胞比例分别为63.80±0.64、33.29±0.21和2.91±0.04,而两对照组鼻咽癌CNE2细胞处于G0/G1期、S期和G2/M期的细胞比例分别为47.91±0.65(44.64±0.28)、42.71±0.11(45.70±0.15)和9.38±0.14(9.65±0.01)。实验组鼻咽癌CNE2细胞的增殖指数为36.2±0.65,而两对照组鼻咽癌CNE2细胞分别为52.09±0.08和55.35±0.08。结论EGFR和IGF-1R基因治疗鼻咽癌机理之一可能是通过改变鼻咽癌CNE2细胞周期趋势和降低移植瘤的增殖水平达到的;PCNA标记指数、核分裂像计数和G2/M期的鼻咽癌细胞比例在一定程度上均可反应鼻咽癌细胞的增殖水平,三者可作为评估鼻咽癌增殖活性的有用指标。Objective To study the cellular proliferation status ografls in nude mice and cell cycle of nasopharyngeal carcinoma cells of human nasopharyngeal carcinoma xen- by dual silencing of IGF-1 and EGF receptors （DSIER）. Methods All the cases were stained with PCNA on paraffin embedded sections by immuno- histochemistry. Mitotic count was done on HE sections corresponding to each cases. Cell cycles of nasopharyngeal cells by DSIER were measured by flow cytometry. The Replication Index（RI） was counted accorrding to cell cycle. Results The mean of PCNA labeling index（ PCNA-LI ） was 27.19 ± 10.20 in DSIER group,while both of control groups were 36.47 ± 8. 63 and 37.07 ± 8. 63, respectively. The mean of mitotic count per 10 high power fieldsis was 18. 80 ± 7.47 in DSIER group, while both of control groups were 37.53 ± 5.25 and 38.03± 6.15, repectively. The correlation coefficient between them was 0. 505 （ P 〈 0.01 ） in DSIER group. There is a significantly positive correlation between PCNA-LI and mitotic counts in DSIER group. The RI in DSIER group was 36.2 ±0.65 ,while RI of both of control groups was 52.09 ±0.08 and 55.35 ±0.08 ,respectively. The cell rates in G0/G1 phase,S phase and G2/M phase of DSIER group were 63.80 ±0.64,33.29±0.21 and 2.91 ± 0.04, respectively, while the cell rates in G0/G1 phase, S phase and G2/M phase of both of control groups were 47.91 ± 0.65 and 9.38 ± 0.14, respectively. Conclusion Dual silencing of IGF-1 and EGF receptors has obvious effect on the PCNA labeling index and mitotic count of human nasopharyngeal carcinoma xenografts in nude mice and on cell cycle of nasopharyngeal carcinoma cells. PCNA labeling index, mitotic count and cell cycle can be used to evaluate the cellular proliferation status of Nasopharyngeal carcinoma.

关 键 词：鼻咽癌 PCNA标记指数 核分裂像计数 免疫组织化学 流式细胞仪 

分 类 号：R739.63[医药卫生—肿瘤]