Syndecan-1和HPA-1 mRNA在胃癌组织中的表达及其与胃癌侵袭转移的关系  

Expression of Syndecan-1 and HPA-1 and their relationship with the invasion and metastasis of gastric carcinoma

作　　者：亓玉琴[1] 司君利[1] 李文利[1] 王贺[1] 周长宏[1] 许琳[1] 

机构地区：[1]青岛大学医学院附属青岛市市立医院消化科,山东青岛266011

出　　处：《中国癌症杂志》2010年第2期110-115,共6页China Oncology

摘　　要：背景与目的:Syndecan-1和HPA-1(乙酰肝素酶-1)可能参与多种恶性肿瘤的侵袭转移,但关于两者与胃癌的关系较少报道。本研究旨在探讨Syndecan-1mRNA和HPA-1mRNA在胃癌组织中的表达及两者与胃癌侵袭转移的关系。方法:采用实时荧光定量逆转录聚合酶链反应(RT-PCR)分别检测了58例胃癌及58例配对的癌旁组织(距癌2cm)和58例配对远离胃癌的手术切缘正常组织(距癌5cm以上)Syndecan-1和HPA-1的mRNA的表达水平,分析探讨两者与胃癌临床病理特征和胃癌侵袭转移的关系。结果:HPA-1mRNA在胃癌中表达的阳性率(86.2%)显著高于癌旁组织(27.6%)和正常组织(5.2%)(P均<0.001),癌旁组织明显高于正常组织(P均<0.05)。Syndecan-1mRNA在正常组织中表达的阳性率(98.3%)显著高于癌旁组织(25.9%)和胃癌组(5.2%)(P均<0.001),癌旁组织中明显高于胃癌组织(P均<0.05)。Syndecan-1的表达减弱和HPA-1的表达增强与肿瘤的分化程度、浸润深度、淋巴结转移、远处转移及TNM分期显著相关(P<0.05)。Syndecan-1mRNA的表达与HPA-1mRNA的表达呈负相关。结论:Syndecan-1mRNA在正常胃组织中呈高表达,HPA-1mRNA在胃癌组织中呈高表达,且Syndecan-1的表达减弱与HPA-1的表达增强可促进胃癌的侵袭转移。联合检测Syndecan-1mRNA及HPA-1mRNA对于指导胃癌临床治疗及判断预后有重要价值。Background and purpose：Syndecan-1 and HPA-1 may be involved in the progression of invasion and metastasis of many malignant tumors, but there are few reports about the relationship between the two gene expressions in gastric carcinomas. This study was aimed to explore the expression of Syndecan-1 and HPA-1 mRNA in gastric carcinoma and their relationship with the invasion and metastasis of gastric carcinoma. Methods： Real-time polymerase chain reation （RT-PCR） was used to detect mRNA of Syndecan-1 and HPA-1 in 58 cases of gastric carcinoma, 58 paired adjacent gastric carcinoma （2 cm from carcinoma）, and 58 surgical marginal normal gastric mucosa tissues （5 cm from carcinoma）. Then we analyzed their relationship with clinico-pathological characteristics of gastric carcinoma. Results：The upregulation of Syndecan-1 mRNA was significantly higher in normal gastric mucosa （98.3%） than that in paired adjacent mucosa （25.9%） and gastric carcinoma （5.2%） （all P〈0.001）.The upregulation of Syndecan-1 mRNA was significantly higher in paired adjacent mucosa than that in gastric carcinoma （all P〈0.05）. The upregulation of HPA-1 mRNA was significantly higher in gastric carcinoma （86.2%） than that in paired adjacent gastric carcinoma （27.6%） and normal gastric mucosa （5.2%） （P〈0.001）. The upregulation of HPA-1 mRNA was significantly higher in paired adjacent gastric carcinoma than that in normal gastric mucosa （all the same P〈0.05）. The downregulation of Syndecan-1 and the upregulation of HPA-1 had relationship with the degree of differentiation, depth of infiltration, lymph node metastasis, distant metastasis and TNM staging of gastric carcinoma （P〈0.05）. Conclusion：The upregulation of Syndecan-1 mRNA was significantly higher in normal gastric mucosa. The upregulation of HPA-1 mRNA was significantly higher in gastric carcinoma. Also, the expression of Syndecan-1 and HPA-1 could predict the invasion and metastasis of gastric carcinoma. Determination of Syn

关 键 词：SYNDECAN-1 HPA-1 胃癌 肿瘤的侵袭和转移 实时荧光定量逆转录聚合酶链反应 

分 类 号：R735.2[医药卫生—肿瘤]