β-连接素和基质金属蛋白酶-2在胃腺癌组织和淋巴结转移灶中的表达及其意义  

Expressions of Beta-Catenin and Matrix Metalloprotein-2 in Gastric Adenocarcinomas and in Metastatic Lymph Nodes

作　　者：田素芳[1] 熊永炎[1] 

机构地区：[1]武汉大学中南医院病理科,湖北武汉430071

出　　处：《武汉大学学报（医学版）》2010年第2期216-219,F0002,共5页Medical Journal of Wuhan University

摘　　要：目的:探讨胃腺癌中β-连接素(β-cat)和基质金属蛋白酶-2(MMP-2)的表达及其与胃腺癌侵袭转移的关系。方法:用SP免疫组化法检测80例胃癌和40例淋巴结转移灶中β-cat和MMP-2的表达。结果:①β-cat在癌旁正常胃黏膜上皮为胞膜表达,而在胃腺癌组织中出现胞膜、质、核三种表达方式,MMP-2在肿瘤细胞中则为胞质表达。②β-cat的表达与胃腺癌的分化程度相关,膜表达随分化程度降低而下降,而其胞质表达随分化程度降低而升高(P<0.05)。β-cat膜表达下降与淋巴结转移状态相关;β-cat胞质表达则在肿瘤原发灶显著高于转移灶(P<0.05)。③MMP-2的表达随肿瘤分化程度下降、浸润深度增加而升高,在有淋巴结转移组高于淋巴结阴性组(P<0.05)。④MMP-2的表达与β-cat的膜表达呈负相关。结论:β-catenin的表达异常导致细胞之间的黏附力下降,MMP-2的表达增高预示肿瘤细胞侵袭能力增强,且两者协同作用,在胃腺癌的侵袭和转移中发挥重要作用。Objective：To investigate the expressions of β-catenin and MMP-2 in gastric carcinomas and their relation with invasion and metastasis. Methods： The expressions of β-catenin and MMP-2 were detected in 80 primary gastric carcinoma tissue samples and in 40 samples of metatastic lymph nodes by means of SP immunohistochemical staining. Results： ①Besides membranous and cytoplasmic staining, nuclear location of β-catenin was also observed in carcinoma tissue. Cytoplasmic MMP-2 staining was common in tumor cells. ②The expression of β-catenin correlated with tumor differentiation. The membranous expression of β-catenin was decreasing along with the increasing in the grade of differentiation, while the cytoplasmic expression of β-catenin was increasing （P0.05, respectively）. Reduction in membranous expression of β-catenin was related to the status of lymph node metastasis （P0.05）. The cytoplasmic expression of β-catenin was higher in primary tumors than in metastases （P0.05）. ③The expression of MMP-2 related to differentiation grade, invasion, and metastasis of tumor （P0.05, respectively）. ④A negative correlation was observed between the expression of MMP-2 and the membranous expression of β-catenin. Conclusion： The anomalous expression of β-catenin leads to reduced cell-cell adhesion, and the increased expression of MMP-2 indicates enhanced invasive ability of tumor cells. The synergistic action between abnormal cell-cell adhesion and matrix degradation plays a pivotal role in gastric cancer cells＇ invasion and metastasis.

关 键 词：胃腺癌 侵袭 转移 Β-连接素 基质金属蛋白酶-2 

分 类 号：R735.2[医药卫生—肿瘤]