不同亚型肾细胞癌的微血管密度与临床病理因素的关系  

Correlation of microvascular density and clinicopathological factors in different subtypes of renal cell carcinoma

作　　者：张艳辉[1] 杨庆[1] 崔巍[1] 刘岩雪[1] 刘素香[1] 姚欣[1] 

机构地区：[1]天津医科大学附属肿瘤医院泌尿外科天津市肿瘤防治重点实验室,300060

出　　处：《中华肿瘤杂志》2010年第2期117-122,共6页Chinese Journal of Oncology

摘　　要：目的探讨CD34和CD31在不同亚型肾细胞癌（RCC）中的表达，及其标记的微血管密度（MVD）与RCC临床病理因素之间的关系。方法采用免疫组化SP法，检测CD34和CD31在149例RCC组织（肾透明细胞癌76例，肾乳头状癌42例，肾嫌色细胞癌31例）中的表达情况。结果肾透明细胞癌、肾嫌色细胞癌和肾乳头状癌组织中CD34标记的MVD分别为128．04±46．44、48．55±14．09和38．12±10．98，CD31标记的MVD分别为98．35±55．05、30．70±17．72和21．604-9．38，肾透明细胞癌组织中CD34标记的MVD和CD31标记的MVD均明显高于肾非透明细胞癌组织（均P〈0．01）。在肾嫌色细胞癌和肾乳头状癌组织中，CD34标记的MVD均明显高于CD31标记的MVD（均P〈0．01）。CD34和CD31标记的MVD均与肾透明细胞癌的组织学分级呈负相关（rCD34=-0．618，P〈0．01；rCD31=-0．442，P〈0．01），也与临床分期呈负相关（rCD34=-0．283，P〈0．05；rCD31=-0．256，P〈0．05），其中CD34的相关性较强；但与肾非透明细胞癌的组织学分级和临床分期无明显的相关性（均P〉0．05）。结论CD34和CD31均能清晰地、选择性地显示RCC的MVD，但CD34比CD31更敏感。肾透明细胞癌的MVD显著高于肾非透明细胞癌。肾透明细胞癌的MVD与组织学分级和临床分期呈负相关，而肾非透明细胞癌的MVD与组织学分级和临床分期并无相关性。Objective The aim of this study was to evaluate the expressions of CD34, CD31 and microvessel density （MVD） in different subtypes of renal cell carcinoma （RCC） as well as the relationship between MVD and clinicopathological factors. Methods Expressions of CD31 and CD34 were detected in 149 patients with RCC using SP immunohistochemical staining. The MVD was studied by Weidner＇s method. Results The expressions of CD31 and CD34 in the clear cell renal cell carcinoma （CCRCC） （98.35± 55.05, 128.04±46.44） were significantly higher than those in chromophobe renal cell carcinoma （ChRCC） （ 30.70 ± 17.72, 48.55 ±14.09） and papillary renal cell carcinoma （PRCC） （21.60±9.38, 38.12 ± 10.98） （P 〈0.01 ）. The MVD value marked by CD31 （30.70 ±17.72, 21.60±9.38） was much lower than that marked by CD34 （48.55 ± 14.09, 38. 12 ± 10.98） between ChRCC and PRCC （P 〈 0.01）. Smaller and immatured microvessels and even single endothelial ceUs could be clearly seen. The MVD values marked by CD31 and CD34 were negatively correlated with the pathological grades （rCD34 = -0. 618, P 〈 0.01 ; rCD31 = -- 0. 442, P 〈 0.01 ） and clinical stages （ reD34 = - 0. 283, P 〈 0.05 ; rCD31 = - 0. 256, P 〈 0.05） in CCRCC. But no association was found in non-CCRCC （ P 〉 0. 05 ）. Conclusion MVD is significantly correlated with different types of endothelial labeling. The microvascular endothelial cells could be shown clearly by its related antigen labeling such as CD34 and CD31. CD34 is more sensitive than CD31. The MVD of CCRCC is significantly higher than that in non-CCRCC. The expressions of CD31 and CD34 are not correlated with tumor grade and stage in ChRCC and PRCC, while there is a negative correlation in CCRCC.

关 键 词：肾肿瘤 微血管密度 临床病理学 

分 类 号：R737.11[医药卫生—肿瘤]