MMP-9、TIMP-1和NF—κB在甲状腺乳头状癌中的表达及意义  

Expression of MMP-9, TIMP-1, and NF-κB in papillary thyroid carcinoma and its significance

作　　者：石建华[1] 汪万英[2] 李阳[3] 

机构地区：[1]蚌埠医学院第一附属医院内分泌科,233004 [2]蚌埠医学院第一附属医院中心实验室 [3]蚌埠医学院病理学教研室

出　　处：《中华内分泌代谢杂志》2010年第2期129-131,共3页Chinese Journal of Endocrinology and Metabolism

基　　金：基金项目：安徽省教育厅资助项目（2006KJ347B）

摘　　要：采用免疫组织化学和原位杂交方法检测157例甲状腺乳头状癌和30例甲状腺乳头状瘤组织中基质金属蛋白酶9（MMP-9）、金属蛋白酶组织抑制因子1（TIMP-1）及NF—tcB的表达。结果显示甲状腺乳头状癌中MMP-9及NF—κB的蛋白及mRNA表达阳性率均明显高于乳头状瘤（均P〈0．01）。MMP-9及NF—κB蛋白和mRNA在甲状腺乳头状癌随着病理分级增高而显著增加（P〈0．01）；并且颈部淋巴结有转移者均高于无转移者（P〈0．01）。而TIMP-1蛋白及mRNA阳性表达率与MMP-9阳性表达率相反。The expressions of matrix metalloproteinase-9 （ MMP-9 ） , tissue inhibitor of metalloproteinase-1 （TIMP-1） ,and NF-κB in 157 cases of papillary thyroid cancer and 30 cases of papillary thyroid tumors were detected by immunohistochemistry and in situ hybridization. The expressions of MMP-9 and NF-κB protein and mRNA in papillary thyroid cancers were significantly higher than those in benign tumors （ all P 〈 0.01 ） , and increased according to the pathological grading（P〈0.01 ）. The expressions of MMP-9 and NF-κB protein and mRNA in papillary thyroid cancers with cervical lymph node metastasis were higher than those without metastasis （ both P 〈 0.01 ）. The expressions of TIMP-1 protein and mRNA appeared an opposite direction of MMP-9 expression.

关 键 词：基质金属蛋白酶9 金属蛋白酶组织抑制因子1 NF-κB 原位杂交 免疫组织化学 

分 类 号：R736.1[医药卫生—肿瘤]