转肿瘤坏死因子-α基因的肿瘤引流淋巴结细胞对人舌鳞癌SCID鼠移植瘤的生长抑制作用  

Inhibition effects of tumor necrosis factor-α gene transduced tumor drainage node of lymphocytes from tongue cancer on SCID mice transplanted tumor established with human tongue carcinoma cell lines

作　　者：孟箭[1] 郭伟[2] 张志愿[2] 任国新[2] 竺涵光[2] 何悦[2] 周晓健[2] 

机构地区：[1]东南大学医学院附属徐州市中心医院口腔科,江苏徐州221009 [2]上海交通大学医学院附属第九人民医院口腔颌面外科,上海200011

出　　处：《华西口腔医学杂志》2010年第1期25-28,共4页West China Journal of Stomatology

基　　金：徐州市社会发展科技基金资助项目(XM08C045)

摘　　要：目的探讨在体外具有强大抗瘤活性的经肿瘤坏死因子-α(TNF-α)基因转导的肿瘤引流淋巴结细胞(DNL)在体内的抑瘤效果以及与平阳星(PYC)联合应用的可行性。方法将舌鳞癌细胞系Tca8113细胞注入SCID鼠背部皮下建立移植瘤模型,取经TNF-α基因转导的DNL细胞在肿瘤区局部注射,联合应用低剂量PYC,观察肿瘤生长情况。至第8周以瘤重计算抑瘤率,并取瘤体标本经处理后作电镜和TUNEL检查,观察细胞凋亡情况。结果TNF/DNL加重组白细胞介素-2(rIL-2)组和TNF/DNL加rIL-2加PYC组抑瘤率均较高,2组间差异有统计学意义(P<0.05);TNF/DNL加rIL-2组超微电镜及TUNEL检测均见凋亡细胞,与对照组相比,凋亡指数(AI)较高(P<0.05)。结论经TNF-α基因转导的DNL局部应用具有明显的抑瘤效果,与PYC联合应用抑瘤效果更大。诱导肿瘤细胞凋亡可能是TNF/DNL抗人舌鳞癌的重要机制之一。Objective To observe the in vivo inhibition effects of tumor necrosis factor-α（TNF-α） gene transduced tumor drainage node of lymphocytes（DNL） from tongue cancer on SCID mice transplanted tumor.Methods 15 human tongue carcinoma models were established in SCID mice by subcutaneously injection of squamous cell carcinoma line Tca8113.TNF-α gene introduced DNL,combined with low dose Pinyancin（PYC）,were locally injected into tumor site.The inhibition rate was determined by the weights at the 8th week after tumor dissection and fresh specimens were prepared and subject to histopathologic examination under transmission electron microscope,and in situ TUNEL was used to detect apoptosis.Results The TNF/DNL and rIL-2 group,and the TNF/DNL and rIL-2 and PYC group both exerted a strong inhibition effect on the implanted tumor.Treated tumors of the TNF/DNL and rIL-2 and PYC group were significantly reduced in comparison with those of the TNF/DNL and rIL-2 group（P 0.05）.The apoptosis of tumor in the TNF/DNL and rIL -2 group was evidenced based on transmission electron microscope and TUNEL analysis,and the apoptosis index was higher than that of control group（P0.05）.Conclusion Local injection of DNL modified with TNF-α gene,combined with low dose PYC,exert a synergistic antitumor effect.Apoptosis may be an important mechanism of squamous cell carcinoma killed by TNF/DNL.

关 键 词：鳞状细胞癌 淋巴细胞 肿瘤坏死因子 

分 类 号：R739.8[医药卫生—肿瘤]