三氧化二砷对黑素瘤B16细胞黏附和迁移的影响  

The influence of arsenic trioxide on adhesion and migration in melanoma B16 cells

作　　者：杨同怀[1] 陈治文[2] 夏俊[2] 王惠[1] 

机构地区：[1]蚌埠医学院临床检验诊断学实验中心,安徽蚌埠233030 [2]蚌埠医学院生物化学与分子生物学教研室,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2010年第3期217-220,共4页Journal of Bengbu Medical College

基　　金：安徽省教育厅自然科学研究资助项目(2006kj351B)

摘　　要：目的:了解三氧化二砷(As2O3)对黑素瘤B16细胞迁移能力的影响,评价As2O3抗肿瘤侵袭和转移能力。方法:用Transwell侵袭转移模型评价As2O3抗黑素瘤B16细胞的侵袭和转移作用。MTT法检测B16细胞的黏附能力。流式细胞术检测B16细胞表面黏附分子CD44的表达情况。结果:As2O3可显著抑制B16细胞的迁移和黏附能力(P<0.05～P<0.01),降低细胞表面黏附分子CD44的表达(P<0.01)。结论:As2O3具有抗黑素瘤B16细胞侵袭和转移作用,降低细胞表面黏附分子CD44的表达可能是其机制之一。Objective：To investigate the influence of arsenic trioxide （As2O3） on migration and adhesion capability in melanoma B16 cells,and evaluate the effect of As2O3 on anti-invasion and anti-metastasis ability. Methods：The invasion and migration capacities of melanoma B16 cells were evaluated using Transwell Permeable Supports. The adhesion capacity of melanoma B16 cells was evaluated by MTF assay. The expression of adhesion molecule CD44 was detected by FCM. Results： As2O3 significantly inhibited the adhesion and migration capacities of melanoma B16 ceils （ P 〈 0.05 to P 〈 0.01 ） and down-regulated the expression level of CD44 （ P 〈 0.01 ）. Conclusions：As2O3 has the effect of antagonizing invasion and metastasis in melanoma B16 cells, the mechanism maybe related to decrease the expression of adhesion molecule CD44.

关 键 词：三氧化二砷 黑素瘤 B16细胞 迁移 黏附实验 CD44 

分 类 号：Q613.63[生物学—生物物理学] R739.5[医药卫生—肿瘤]