高效液相色谱-质谱法测定发酵液中的喷司他丁  

Determination of pentostatin in culture broth using high performance liquid chromatography-mass spectrometry

作　　者：杨鹏[1] 王岩[1] 廖艳艳[1] 

机构地区：[1]上海医药工业研究院,上海200040

出　　处：《色谱》2010年第3期316-318,共3页Chinese Journal of Chromatography

基　　金：上海医药工业研究院"重大新药创制"科技重大专项项目(2009zx09301-007)

摘　　要：建立了测定发酵液中喷司他丁含量的反相高效液相色谱-质谱分析方法。采用的色谱条件:色谱柱为Hyper-silODS2柱(250mm×4.6mm,5μm);流动相为甲醇/乙腈/10mmol/L乙酸铵(pH7.6)(2.5/2.5/95,v/v/v),流速为1.0mL/min;检测波长为280nm;柱温为40℃;进样量为10μL。喷司他丁在1.0～100mg/L范围内有良好的线性关系,相关系数为0.9999。该方法精密度好,稳定性高,能简便、快速、准确地测定发酵液中喷司他丁的含量。A method for the determination of pentostatin in culture broth was established using high performance liquid chromatography-mass spectrometry （HPLC-MS）. The chromatographic conditions used were as follows： the analytical column was Hypersil ODS2 （250 mm× 4.6 ram, 5 μm） ; the mobile phase was methanol/acetonitrile/10 mmol/L ammonium acetate （pH 7.6） （2.5/2.5/95, v/v/v） with a flow rate of 1.0 mL/min; the detection wavelength was 280 nm; the injection volume was 10 μL; the temperature was 40 ℃ The linear range of the method for pentostatin was 1.0 - 100 mg/L （r = 0. 999 9）. The method is characterized by simplicity, higher sensitivity and accuracy.

关 键 词：高效液相色谱-质谱法 喷司他丁 发酵液 

分 类 号：O658[理学—分析化学]