基于不同引物的RT-PCR检测IBDV敏感性的比较  

The sensitivity of RT-PCR based on two pairs of primers for the detection of infectious bursal disease virus (IBDV)

作　　者：王艳[1] 李树清[1] 孙建和[2] 汪振兴[2] 陈志飞[1] 张强[1] 王巧全[1] 严亚贤[2] 

机构地区：[1]上海出入境检验检疫局,上海200135 [2]上海交通大学,上海201101

出　　处：《中国动物检疫》2010年第3期32-36,共5页China Animal Health Inspection

基　　金：上海市科委标准专项项目(06DZ05033);上海出入境检疫检验课题(HK078-2007)资助

摘　　要：根据GenBank中鸡传染性法氏囊病病毒(IBDV)A片段保守区域设计、合成了一对特异性引物(RP1/RP3),以期建立特异、灵敏的IBDVRT-PCR检测方法。结果显示RP1/RP3具有良好的特异性和重复性;将之与基于OIE推荐引物(L2/U2)建立的RT-PCR检测方法进行比较,发现基于引物RP1/RP3检测IBDV的灵敏度明显高于L2/U2,特别是当待检样品中的IBDV的含量低时,会避免因使用L2/U2导致出现漏检的现象。结论:以RP1/RP3作为引物建立的IBDVRT-PCR检测方法灵敏性高、特异性强,可用于IBDV的检疫、诊断和监测。An RT-PCR method was developed for the detection of infectious bursal disease virus （IBDV） using a pair of primers designed according to the gene of A segment of IBDV. A comparison of the detection sensitivity based on these two primer pairs （RP1/RP3, L2/U2） for IBDV was carried out using RT-PCR. The results showed that the sensitivity of RP1/RP3 was much higher than that of L2/U2, especially when IBDV is extremely low in the samples, it is essential to choose much more sensitive primers in order to avoid false negative results. The assay developed here is highly specific and has no cross reactivity with other poultry virus, and it is a convenient tool for detection of IBDV.

关 键 词：鸡传染性法氏囊病毒 引物 RT-PCR 敏感度 

分 类 号：S852.659.4[农业科学—基础兽医学]