乌头类生物碱对ras基因表达影响及其抗肿瘤分子机制研究  

STUDY ON THE EFFECT OF ACONITIC ALKALOID ON RAS GENE EXPRESSION AND ITS MOLECULAR MECHANISM OF ANTITUMOR ACTIVITY

作　　者：饶朝龙[1] 彭成[1] 

机构地区：[1]成都中医药大学,成都610075

出　　处：《现代预防医学》2010年第6期1098-1100,1103,共4页Modern Preventive Medicine

基　　金：国家重点基础研究发展计划(2006CB504705);国家自然科学基金重点项目(30230410)

摘　　要：[目的]研究乌头类生物碱对细胞增殖周期和ras基因表达的影响,探讨乌头类中药抗肿瘤作用的分子机制。[方法]体外培养大鼠视网膜神经细胞,分别以0.5%的乌头碱、新乌头碱和次乌头碱作用细胞5min后,流式细胞仪检测。[结果]乌头碱、新乌头碱和次乌头碱作用下,细胞增殖活动被抑制,ras基因表达量显著下降。[结论]ras基因及Ras/Raf/MEK/MAPK信号级联通路可能是乌头类中药抗肿瘤作用的重要靶点。[Objective] To study the effect of aconitic alkaloid on cell cycle and ras gene expression, and explore its molecular mechanism of anti-tumor activity. [Methods] Rat retina neuron was cultivated in vitro, and treated with aconitine, mesaconitine and hypaconitine for 5 minutes respectively, then determined with Flow Cytometer. [Results] With the effect of aconitine, meaconitine and hypaconitine, cell proliferation decreased significantly, and amount of ras gene expression decreased significantly. [Conclusion] ras gene and Ras / Raf / MEK / MAPK signal pathway should play an important role in the molecular mechanism of aconite’s anti-tumor activity.

关 键 词：乌头类生物碱 RAS基因 Ras/Raf/MEK/MAPK信号通路 

分 类 号：R735.2[医药卫生—肿瘤]