家兔肠缺血—再灌注损伤时脂质过氧化的变化及其意义  

CHANGES AND SIGNIFICANCE OF LIPID PEROXIDATION ON BOWEL ISCHEMIA REPERFUSION INJURY IN RABBITS

作　　者：张硕森[1] 李宏杰[1] 景有伶[1] 张连元[1] 崔和勤[2] 刘红[3] 夏桂花 

机构地区：[1]华北煤炭医学院病理生理教研室 [2]华北煤炭医学院生物化学教研室 [3]华北煤炭医学院医用化学教研室

出　　处：《天津医科大学学报》1998年第4期325-327,共3页Journal of Tianjin Medical University

基　　金：煤炭科学基金

摘　　要：目的：探讨肠缺血－再灌注（Ｉ／Ｒ）损伤时血浆及组织脂质过氧化的变化及其意义。方法：复制家兔肠Ｉ／Ｒ损伤模型，检测超氧化物歧化酶（ＳＯＤ）和黄嘌呤氧化酶（ＸＯ）活性及丙二醛（ＭＤＡ）含量。实验分为Ｉ／Ｒ损伤组及假手术（Ｓｈａｍ）对照组，并进行比较。结果：缺血前两组动物ＳＯＤ、ＸＯ及ＭＤＡ值均无统计学差异，Ｉ／Ｒ组再灌注后２ｈ与缺血前及Ｓｈａｍ组相比，ＳＯＤ活性显著下降，ＸＯ活性及ＭＤＡ含量明显增加（Ｐ〈０．０１）；此外，肺、肝等组织ＭＤＡ含量亦明显高于Ｓｈａｍ组（Ｐ〈０．０１）。结论：家兔肠Ｉ／Ｒ损伤时体内脂质过氧化过程加强，且在Ｉ／Ｒ损伤过程中起重要作用。Objective: To investigate the changes and significance of lipid peroxidation on bowel ischemia reperfusion(I/R)injury in rabbits Methods:The models of rabbit bowel I/R injury was made The changes of the activity of superoxide dismutase (SOD),xanthine oxidase(xo)and malondialdehyde(MDA) were determined Results: SOD activity was markedly decreased and XO activity and MDA content were markedly increased 2h after reperfusion Copared with before ischemia and sham group( P <0 01);The MDA content in lung and liver tissues etc were significantly higher than that in sham group( P <0 01) Conclusions: It is suggested that the lipid peroxidation process was increased in bowel I/R injury and might play an important role in I/R injury process

关 键 词：肠缺血 再灌注损伤 脂质过氧化 SOD 

分 类 号：R574.02[医药卫生—消化系统]