脑出血大鼠脑内NGF和BDNF蛋白表达变化的研究  

The Expressions of NGF and BDNF in the Rats with Intracerebral Hemorrhage

作　　者：石秋艳[1] 何俊芳[1] 孙惠芳[1] 孙鹏[2] 刘斌[1] 张国志[1] 王长友[1] 燕霞[3] 

机构地区：[1]华北煤炭医学院附属医院,063000 [2]唐山市铁路中心医院,063000 [3]河北工程大学附属医院,056002

出　　处：《中国医药指南》2010年第7期8-10,共3页Guide of China Medicine

摘　　要：目的观察脑出血大鼠脑组织内神经生长因子(NGF)和脑源性神经营养因子(BDNF)表达的变化,探讨神经营养因子在脑出血后脑损伤的作用。方法SD雄性大鼠随机分为正常组、假手术组和脑出血组,通过自体血脑内注射法建立脑出血动物模型,采用免疫组织化学法检测脑出血后3、6、12、24、48、72h、7d脑组织中NGF和BDNF蛋白表达情况。结果脑出血组6hNGF表达增加,12hBDNF表达增加,均于24h达高峰,与假手术组及正常组相比差异具有显著性(P<0.05)。结论脑出血后可诱导NGF和BDNF蛋白表达增加,有利于对脑出血后损伤神经元的保护和修复。Objective To observe the expression on distribution of NGF and BDNF in rat brain with intracerebral hemorrhage, in order to explore the fuction of the neurotrophic factors against intracerebral hemorrhage injury. Methods Adult male Sprague-Dawle rats were randomly seperated into three groups： normal group, sham operation group, intracerebral hemorrhage group, then the model of intracerebral hemorrhage（ICH） was established in rats by intraeerebral injection with autogenous blood. The protein expression of BDNF and NGF was detected by immunohisto-chemistry at 3, 6, 12, 24, 48, 72h, 7d after intracerebral hemorrhage. Results The NGF expression was found increasing in the perienchyma at 6h and BDNF expression increasing in 12h, both peaking at 24h after hemorrhage. Comparing with normal group and sham operation group, the increases of the indexes of BDNF and NGF were statistically significant（P〈0.05）. Conclusion The expression of NGF and BDNF increases as the inducement in the brain of rats after intracerebral hemorrhage, and it is beneficial for the protection and repair of brain neurons.

关 键 词：脑出血 脑源性神经营养因子 神经生长因子 

分 类 号：R743.34[医药卫生—神经病学与精神病学]