细菌纤维素构建组织工程角膜基质的方法及其评价  

Method to construct tissue engineering corneal stroma with bacterial cellulose and its evaluation

作　　者：贾卉[1] 贾原媛[2] 王娇[1] 胡源[6] 张媛 周余来[4] 王苹[5] 

机构地区：[1]吉林大学第一医院眼科,吉林长春130021 [2]天津科技大学材料科学与化学工程学院,天津300457 [3]日本Ehime大学眼科,日本790-0925 [4]吉林大学药学院生物工程教研室,吉林长春130021 [5]吉林大学第一医院耳鼻喉科研究所,吉林长春130021 [6]南方医科大学临床医学

出　　处：《吉林大学学报（医学版）》2010年第2期303-307,I0003,共6页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科技发展计划项目资助课题(200905136)

摘　　要：目的:探讨以细菌纤维素(BC)为支架构建组织工程角膜基质的可行性。方法:体外分离培养兔和人角膜基质细胞,种植到BC膜中,构建完成后进行兔和人角膜基质细胞-BC复合膜的生物检测,并将兔角膜细胞生物复合物进行同种异体移植。术后1、4和8周时分别活体行前节OCT、角膜共焦显微镜检查,离体后组织学及免疫组织化学检查。结果:人和兔角膜基质细胞长入BC的网架结构,细胞生长状态良好。移植术后1周兔眼无炎症反应及新生血管,4周后植片边缘出现新生血管,表面无水肿及溃疡形成。术后8周角膜共焦显微镜显示:移植片周围基质细胞增生活跃,邻近的角膜内皮细胞数量和形态正常。前节OCT显示:移植后复合膜逐渐降解,4周时复合膜边界模糊,8周时密度接近正常角膜组织。离体组织学检查显示:BC复合膜与正常角膜基质相似,有炎细胞浸润和血管形成,角膜组织无坏死和溶解。结论:BC无细胞毒性,具有良好的组织相容性和可降解性,可作为构建组织工程角膜基质的生物支架。Objective To investigate the potentiality of bacterial cellulose（BC） as the scaffold of tissue engineering corneal stroma.Methods Rabbit and human corneal stromal cells were separated and seeded into a BC membrane.The compound membrane with rabbit stroma cells was allogeneil grafted and examined by corneal confocal microscopy,anterior OCT and histological method and immunohistochemical staining 1,4 and 8 weeks after operation.Results The rabbit and human corneal stromal cells grew well in the BC membrane.One week after grafting,the inflammatory reaction and new vessels were not observed in the cornea.After four weeks,the new vessels could be found around the membrane without edema and ulcer on the surface.The rest cornea was still transparent.The stromal cells proliferated actively around the membrane observed by corneal confocal microscopy 8 weeks after transplantation.The quantity and appearance of proximalis corneal endothelium were normal.Anterior OCT displayed that BC compound membrane degraded gradually.The boundary between membrane and normal corneal tissue was vague 4 weeks after operation and similar to normal cornea in density 8 weeks after operation.BC compound membrane approached to normal corneal stromal tissue by ex vivo histology.The inflammatory cell infiltration and new vessels could be detected without necrosis or dissolution.Conclusion BC has a good biocompatibility and degradation without cytotoxicity.It can be used as a biological scaffold for tissue engineering corneal stroma.

关 键 词：角膜基质细胞 细菌纤维素 组织工程 

分 类 号：Q813[生物学—生物工程] R77[医药卫生—眼科]