多药耐药相关蛋白-2在白血病耐药细胞株K562/A02的表达  

Expression of multi-drug resistance relative protein-2 in leukemia multi-drug resistance cell line of K562/A02

作　　者：姜振宇[1] 许颖[2] 荆蕾[3] 姚程[3] 马宁[1] 

机构地区：[1]吉林大学第一医院风湿科,吉林长春130021 [2]吉林大学第一医院肾病内科,吉林长春130021 [3]吉林大学第一医院血液肿瘤科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2010年第2期312-315,共4页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科研基金资助课题(200705140)

摘　　要：目的:研究多药耐药相关蛋白-2(MRP-2)与白血病细胞耐药的关系,并探讨其在白血病多药耐药中的意义。方法:应用RT-PCR方法检测白血病细胞株K562与耐药细胞株K562/A02中MRP-2mRNA的差异表达,然后将MRP-2反义及正义寡核苷酸片段分别用脂质体转染至耐药细胞株K562/A02,采用MTT法、流式细胞术及RT-PCR法检测转染后细胞内阿霉素的荧光强度、耐药指数及MRP-2mRNA的表达情况。结果:耐药细胞株K562/A02中MRP-2mRNA表达水平明显高于白血病细胞株K562(P<0.05)。MRP-2反义寡核苷酸片段转染后耐药细胞株K562/A02细胞内的阿霉素浓度升高,耐药指数较未转染细胞明显降低(P<0.05);转染后耐药细胞株K562/A02细胞MRP-2mRNA表达水平较未转染细胞下降了67.5%,两者比较差异具有显著性(P<0.05)。结论:MRP-2的高表达导致白血病耐药细胞内化疗药物浓度降低,可能是其导致白血病多药耐药的机制之一。Objective To investigate the relationship between multi-drug resistance relative protein-2（MRP-2） and leukemia multi-drug resistance,and study its effect on leukemia multi-drug resistance.Methods The expressions of MRP-2 in leukemia cell line K562 and leukemia multi-drug resistance cell line K562/A02 were detected by PT-PCR.Anti-and oligonucleotide of MRP-2 were transfected by lipidosome into K562/A02,intra-cellular doxorubicin（ADM） fluorescence intensity,resistance index,and the expression of MRP-2 mRNA were detected by MTT,flow cytometry,and RT-PCR.Results The expression of MRP-2 in K562/A02 was higher than that of K562（P〈0.05）.After transfected with anti-oligonucleotide MRP-2,the ADM fluorescence intensity of K562/A02 was increased,and the resistance index was significantly decreased,and the expression of MRP-2 mRNA was decreased by 67.5%（P〈0.05） compared with untransfected cells.Conclusion The high-level expression of MRP-2 results in the reducing of chemical drug concentration,which may be one of the mechanisms of leukemia multi-drug resistance.

关 键 词：白血病 多药耐药 多药耐药相关蛋白-2 

分 类 号：R733.7[医药卫生—肿瘤]