20(s)-原人参二醇诱导体外培养Siha细胞凋亡的作用  

Effect of 20(s)-proto-panaxdiol on apoptosis of Siha cells in vitro

作　　者：赵丽晶[1] 许多[1] 梁作文[1] 程宏[1] 郭亚雄[1] 李德隆[1] 韩向北[1] 赵丽娟[1] 董妍[2] 

机构地区：[1]吉林大学基础医学院病理生理学教研室,吉林长春130021 [2]美国杜兰大学细胞生物学教研室,美国新奥尔良la70062

出　　处：《吉林大学学报（医学版）》2010年第2期345-348,I0005,F0003,共6页Journal of Jilin University：Medicine Edition

基　　金：吉林省人民政府人才开发基金资助课题(2006JD02)

摘　　要：目的:观察20(s)-原人参二醇(PPD)对体外培养人宫颈癌Siha细胞凋亡的作用,阐明其抗肿瘤作用机制。方法:体外培养人宫颈癌Siha细胞,将其分为阴性对照组(乙醇)、阳性对照组(40μg.L-1顺铂)及PPD10、20和40μmol.L-1组。分别用乙醇、顺铂和PPD处理细胞48h后,利用光学显微镜观察细胞形态学改变,采用AnnexinV-EGFP/PI双染色法、TUNUL染色法检测细胞凋亡情况,分别采用RT-PCR、Westernblotting方法检测bax基因的转录及蛋白表达情况。结果:不同剂量的PPD处理后,Siha细胞变圆回缩并出现碎片,AnnexinV-EGFP/PI染色呈现不同程度的绿染和红染,TUNUL染色可见绿色荧光,bax基因的转录与蛋白表达升高。结论:PPD可诱导人宫颈癌Siha细胞出现凋亡,其作用与bax基因表达上调有关。Objective To study the effect of 20（s）-proto-panaxdiol（PPD）on apoptosis of Siha cells in vitro,and clarify the mechanism of its anticancer action.Methods Siha cells cultivated in vitro were treated with alcohol,40 μg·L^-1 Platinol（Pt）,10 μmol·L-1 PPD,20 μmol·L-1 PPD,40 μmol·L^-1 PPD for 48 h,respectively.The morphological changes of Siha cells were observed under optical microscope.Apoptosis was detected by Annexin V-EGFP /PI staining and TUNUL staining.The transcription and the protein expression of bax were analyzed by RT-PCR and Western blotting.Results After treated with PPD,the cells were round,recovery,and broken.Green and red fluorescence was shown in Annexin V-EGFP/PI staining,green fluorescence was evident in TUNUL staining.The transcription and the protein expression of bax were up-regulated.Conclusion PPD may induce the apoptosis of Siha cells,and its mechanism may be related to the up-regulation of bax expression.

关 键 词：20(S)-原人参二醇 宫颈肿瘤 SIHA细胞 细胞凋亡 

分 类 号：Q75[生物学—分子生物学] R737.3[医药卫生—肿瘤]