TNXB基因多态性与精神分裂症发病的遗传学关联性分析  

Association analysis on TNXB gene polymorphisms and schizophrenia in Chinese Han population

作　　者：王姣艳[1,2] 张丽[2] 吴江[3] 刘扬[1] 王珍琦[1] 关松磊[1] 油洋[1] 张萱[1,3] 

机构地区：[1]吉林大学公共卫生学院基因组医学研究中心卫生部放射生物学重点实验室,吉林长春130021 [2]吉林大学第一医院心理卫生科,吉林长春130021 [3]吉林大学第一医院神经内科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2010年第2期359-363,共5页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(30700257);教育部留学回国人员启动基金资助课题(2006);吉林大学创新基金资助课题(200CX)

摘　　要：目的:阐明TNXB基因多态性与中国北方汉族人群精神分裂症发病及其临床表型的关系。方法:收集779例中国北方汉族精神分裂症患者和983例健康人对照组全血样品,从中提取基因组DNA,采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)的方法,检测TNXB基因rs204887位点基因型。应用拟合优度χ2检验分析基因型频数分布是否符合Hardy-Weinberg平衡定律,应用χ2检验和数量性状分析分别进行等位基因、基因型以及各种临床表型的关联性分析。结果:TNXB基因rs204887位点的基因型频数分布在精神分裂症病例组和对照组均符合Hardy-Weinberg平衡定律(P>0.05)。等位基因关联分析,C和T等位基因在病例组和对照组中的频数分布比较差异无显著性(P>0.05);基因型关联分析,C/C、C/T和T/T3种基因型在病例组和对照组的频数分布比较差异无显著性(P>0.05);等位基因、基因型频数分布与精神分裂症各种临床表型均无关联(P>0.05)。结论:TNXB基因rs204887位点可能与中国北方汉族人群精神分裂症发病无关联。Objective To elucidate the genetic association between TNXB gene polymorphisms and schizophrenia in a Han descent population in the north of China.Methods Genomic DNA was isolated from the whole blood samples.A single nucleotide polymorphism,rs204887（Rsa I site） present in the TNXB gene,was detected using PCR-based restriction fragment length polymorphism analysis among 779 Chinese Han patients with schizophrenia and 983 ethnicity-matched healthy controls.The Hardy-Weinberg equilibrium for genotypic distribution was estimated by the goodness-of-fit χ^2 test.The frequencies of alleles and genotypes of TNXB gene and the relationship between allelic frequencies and the clinical phenotypes of schizophrenia were statistically computed.Results The goodness-of-fit χ^2 test result showed that the genotypic distributions of rs204887 did not deviate from Hardy-Weinberg equilibrium in both patient group and control group（P〉0.05）.The χ2 test result did not show allelic association and genotypic association for rs204887（P〉0.05）.There were no correlations between allelic or genotypic frequencies and the clinical phenotypes of schizophrenia（P〉0.05）.Conclusion TNXB locus may not be associated with schizophrenia in a Han descent population in the north of China.

关 键 词：精神分裂症/遗传学 多态性 单核苷酸 TNXB 

分 类 号：Q75[生物学—分子生物学] R749.3[医药卫生—神经病学与精神病学]