黄淮冬麦区不同时期主推品种淀粉合成酶基因分子标记鉴定  

Analysis of Starch Synthesis Genes in Major Wheat Cultivars Grown in Huanghuai Wheat Production Area at Different Periods Using Molecular Markers

作　　者：张瑞奇[1] 胡琳[2] 王秀娥[1] 张守忠[1] 马燕欣[1] 陈佩度[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室/细胞遗传所,南京210095 [2]河南省农科院小麦研究中心,郑州450002

出　　处：《植物遗传资源学报》2010年第2期200-205,共6页Journal of Plant Genetic Resources

基　　金：国家高新技术研究发展计划(863计划)(2006AA10Z1F6)

摘　　要：利用普通小麦直链淀粉合成酶GBSS基因及支链淀粉关键合成酶SSⅡ基因的特异分子标记鉴定了黄淮冬麦区自20世纪50年代以来253份主推品种,发现8份品种缺失Wx-B1基因,其中5份材料(小偃168、秦麦1号、83S502、山东935031、山东928802)是新发现的缺失Wx-B1基因的小麦品种,未发现Wx-A1、Wx-D1基因缺失类型品种;所鉴定253份品种的SSⅡ基因均为野生型,未发现缺失突变类型。通过对8份缺失Wx-B1基因品种直链淀粉含量分析,发现这些品种的直链淀粉含量差异较大,变异范围为19.9%～33.0%,其中豫麦47、83S502和中育5号3个品种的直链淀粉含量较低,分别为19.9%、21.3%和26.4%,可以用于优质面条小麦品质改良。A total of 253 major wheat cuhivars grown in Huanghuai wheat production area at different periods were screened for the starch synthesis genes by molecular markers. The results indicated that 8 of these euhivars were null at Wx-B1, and they were used to identify the amylose content. The results showed that significant variation was observed. The range of amylose content was 19.9% -33.3% , only Yumai 47,83S502 and Zhongyu 5 with null Wx-B1 was below 27%. The 253 wheat cuhivars were also screened for the SS U gene mutations with three STS specific markers published in previous researches. The results indicated that 253 wheat cuhivars were all wild type.

关 键 词：淀粉合成酶基因 分子标记 直链淀粉含量 

分 类 号：S512.1[农业科学—作物学]