大豆种质对SMV成株和种粒斑驳抗性的SSR标记辅助鉴定  

Identification of the SMV Adult-Plant and Seed Coat Mottling Resistance in Soybean Germplasms Using SSR Markers

作　　者：李文福[1,2] 朱晓双[1,2] 王晓锋 王堃[1,2] 刘春燕[1,2] 陈庆山[1,2] 胡国华[1,4] 

机构地区：[1]黑龙江省农垦科研育种中心,哈尔滨150090 [2]东北农业大学农学院,哈尔滨150030 [3]黑龙江省建三江农业科学研究所,富锦156300 [4]国家大豆工程技术研究中心,哈尔滨150050

出　　处：《植物遗传资源学报》2010年第2期239-243,共5页Journal of Plant Genetic Resources

基　　金：国家重点基础研究发展计划(973计划)项目(2004CB117203-5);引进国际先进农业科学技术计划(948计划)项目(2006-G1(A));国家高技术研究发展计划(863计划)项目(2006AA100104-3);国家自然科学基金(30871551)

摘　　要：对186份大豆种质资源进行了成株和种粒斑驳抗性鉴定,并利用与成株抗性及种粒斑驳抗性分别相关的SSR标记验证抗病毒分子辅助选择的可行性。结果表明:接种SMV1,选出成株和种粒双抗种质38份,成株抗病种质149份,种粒抗病种质45份,成株和种粒双感种质26份。利用与成株抗性相关的SSR标记Sat_229、Sat_317、Satt335、Satt160、Satt516、Sat_309进行检测,抗病毒资源筛选的准确率分别达到68.9%、74.3%、71.1%、69.8%、77.4%和68.2%。利用与种粒斑驳抗性相关的SSR标记Sat_297、Sat_229、Sat_317、Satt335、Sct_188、Satt160、Satt516、Sat_133进行检测,Sat_317标记准确率达79.1%,标记Sat_229、Satt335、Satt516和Sat_133抗病毒资源筛选的准确率均达70%以上,可以用作抗病毒分子辅助育种的选择标记。One hundred and ei coat mottling by the inoculation of ghty six soybean germplasms were screened for resistance to adult-plant and seed strain SMV1, and the coincident rate between adult-plant resistance, seed coat mottling resistance to SMV and linkage SSR markers were evaluated. The results showed that among these 186 germplasms,38 were resistant to both adult-plant and seed coat mottling;149 were only resistant to aduh-plant;45 were only resistant to seed coat mottling;and 26 were both susceptible to adult-plant and seed coat mottling. 6 SSR markers, Sat_229, Sat_317, Satt335, Satt160, Satt516 and Sat_309, related to SMV adult-plant resistance, were used to identify the 186 soybean germplasms. The coincident rates between the SSR alleles and the resistant presence were 68.9 % ,74.3 % , 71.1%, 69.8 % , 77.4 % and 68.2% , respectively ; 8 SSR markers, Sat_297, Sat_229, Sat_317, Satt335,Sct_188,Satt160, Satt516 and Sat_133 ,related to SMV seed coat mottling resistance,were used to identify the 186 soybean germplasms,the coincident rates of Sat_317 alleles with the resistant presence were 79.1%, and that of Sat_229, Satt335, Satt516 and Sat_133 were all over 70%. These markers related to SMV1 resistance were available for the marker assisted selection for SMV resistance breeding.

关 键 词：大豆花叶病毒 种质资源 种粒斑驳 分子标记 辅助选择 

分 类 号：S565.1[农业科学—作物学]