TNF-α诱导宫颈癌细胞增殖、转移和耐药相关基因表达的时效分析  

Time effects of TNF-α on proliferation,metastasis and drug resistance related gene expression in cervical cancer Hela cells

作　　者：缪丽芳[1] 陆丹[1] 汪泱[2] 刘丝荪[3] 郭菲[4] 

机构地区：[1]南昌大学研究生院 [2]南昌大学第一附属医院泌尿外科研究所,330006 [3]南昌大学第一附属医院妇产科,330006 [4]南昌大学第一附属医院烧伤研究所,330006

出　　处：《实验与检验医学》2010年第1期21-24,共4页Experimental and Laboratory Medicine

基　　金：国家自然科学基金资助项目(30660182;30960405);江西省自然科学基金(2007GZY1132);江西省卫生厅科技计划(20071041)

摘　　要：目的探讨肿瘤坏死因子(TNF-α)对宫颈癌Hela细胞增殖、转移和化疗耐药相关基因表达的影响。方法以外源性TNF-α作用于宫颈癌Hela细胞,作用不同时间点(0-24h)取样分析细胞周期蛋白cyclinD1,锌指蛋白A20,钙调神经磷酸酶CaN,基质金属蛋白酶(MMP-1,MMP-9),金属硫蛋白(MT-1,MT-2)等表达的时间-效应。结果TNF-α作用宫颈癌Hela细胞不同时间段(20min,1h,6h,24h),cyclinD1、A20、CaN、MMP-1、MMP-9、MT-1、MT-2 mRNA的表达都有不同程度改变。RT-PCR检测宫颈癌Hela细胞经TNF-α作用后cyclinD1、MMP-1、MT-2 mRNA的表达6小时达高峰24小时回落,A20、CaN、MMP-9、MT-1 mRNA表达1小时达高峰24小时回落。结论TNF-α诱导宫颈癌Hela细胞cyclinD1、A20、CaN、MMP-1、MMP-9、MT-1、MT-2 mRNA的表达具有时效性。TNF-α可能促进宫颈癌Hela细胞增殖、转移及耐药。Objective To investigate the effects of tumor necrosis factor-α（TNF-α） on prollieratlon,metastasis and drug resistance-related gene of cervical cancer Hela cells. Methods Hela cells were stimulated by exogenous TNF-α at different time points （0-24h）, then gene expressions of cyclinD1, A20, calcineurin （CAN）, matrix metalloproteinase （MMP-1,MMP-9）, metallothionein （MT-1,MT-2） were detected at mRNA level by reverse transcription-polymerase chain reaction （RT-PCR）. Results At different time points post-TNF-α stimulation （20m, 1h, 6h, 24h）, the expression of cyclinD1, A20, CaN, MMP-1, MMP-9, MT-1, MT-2 mRNA changed in varous degree. After Hela cells were stimulated by exogenous TNF-α, the expression of cyclinD1 ,MMP-1, MT-2 mRNA reached the peak at 6h and fell back at 24h, and the expression of A20, CaN, MMP-9, MT-1 mRNA reached the peak at lh and fell back at 24h. Conclusions TNF-α-induced expressions of cyclinD1, A20, CaN, MMP-1, MMP-9, MT-1 and MT-2 mRNA in Hela cells is time-dependent, which suggests that TNF-α may be able to promote cervical cancer cells proliferation, metastasis and drug resistance.

关 键 词：宫颈癌 肿瘤坏死因子-Α 增殖 转移 耐药 基因 

分 类 号：R737.33[医药卫生—肿瘤] R446.8[医药卫生—临床医学]