高表达Mcl-1对衣霉素诱导胃腺癌细胞凋亡的影响  

Effects of overexpressed Mcl-1 in apoptosis induced by the ER stress inducers of gastric adenocarcinoma cells

作　　者：吴亚欧[1] 张林杰[1] 张旭东[1] 余宏伟[1] 

机构地区：[1]安徽医科大学免疫学教研室,合肥230032

出　　处：《安徽医科大学学报》2010年第1期20-23,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:30572118);安徽省自然科学基金(编号:070413077)

摘　　要：目的探讨Bcl-2蛋白家族中Mcl-1在衣霉素诱导胃腺癌细胞内质网应激性凋亡中的作用。方法采用流式细胞仪PI单染法检测细胞凋亡率;Westernblot检测Bcl-2、Mcl-1、Bcl-XL在衣霉素诱导胃腺癌细胞内质网应激状态下的表达;脂质体LipofectamineTM2000转染pcDNA3.0/Mcl-1质粒;Westernblot分别检测转染前后Mcl-1蛋白的表达水平变化。结果衣霉素诱导胃腺癌细胞凋亡具有剂量和时间依赖性,但两株细胞对衣霉素诱导的凋亡都不敏感,其中SGC-7901较BGC-823对衣霉素的耐受表现更明显。由于衣霉素诱导SGC-7901和BGC-823的凋亡与线粒体凋亡途径有关,检测Bcl-2蛋白家族Mcl-1在SGC-7901中有明显上调。通过转染质粒高表达Mcl-1蛋白,衣霉素诱导的凋亡有明显下降。结论Mcl-1在衣霉素诱导胃腺癌细胞凋亡中发挥重要的抑制作用。Objective To study the role of antiapoptosis protein Mcl-1 in the pathway of apoptosis of ER stress-induced gastric adenocarcinoma cells.Methods Apoptosis was measured by flow cytometry using propidium iodide staining.Changes of Bcl-2、Mcl-1、Bcl-XL were detected by Western blot method.The eukaryotic expression vector pcDNA3.0/Mcl-1 containing human Mcl-1 cDNA and vector pcDNA3.0 were transfected into the resistant SGC-7901 cells,respectively.Western blot was used to detect the target gene expression at protein levels.Results ER stress induced apoptosis with tunicamycin of gastric adenocarcinoma cells in a dose-and time-dependent manner.It appeared that SGC-7901 cells could resist to tunicamycin-induced apoptosis more obviously than BGC-823 cells.The expression of Mcl-1 was increased in SGC-7901.Over-expression Mcl-1 by trasfection protects gastric adenocarcinoma cells from tunicamycin-induced apoptosis.Conclusion Antiapoptosis protein Mcl-1 plays an important role in apoptosis induced by the ER stress inducers of gastric adenocarcinoma cell.

关 键 词：胃肿瘤 细胞凋亡 衣霉素 转染 

分 类 号：R735.2[医药卫生—肿瘤] R978.12[医药卫生—临床医学]