血管内皮生长因子在围植入期小鼠子宫内膜中的表达  

Expression of vascual endothelial growth factor in the peri-implanation mouse endometrium

作　　者：高晓妹[1,2] 陈晓蓉[1] 张荣宜[1] 

机构地区：[1]安徽医科大学组织学与胚胎学教研室 [2]安徽医科大学第一临床学院,合肥230032

出　　处：《安徽医科大学学报》2010年第1期31-34,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省人事厅重点基金(编号:KJ2007A034)

摘　　要：目的观察血管内皮生长因子(VEGF)在围植入期小鼠子宫内膜的表达分布状况和规律。方法应用免疫组化SP法检测妊娠3～6d昆明小鼠围植入期子宫内膜VEGF的表达情况,并以动情期未孕小鼠子宫做对照。结果在小鼠子宫内膜中可检测到VEGF的表达,其表达具有时间特异性和细胞特异性。孕3d,VEGF在子宫内膜的腔上皮和基质细胞中呈弱表达;孕4～5d,VEGF在基质细胞和腔上皮上表达最强;孕6d,VEGF表达较强,但主要表达于蜕膜细胞。结论围植入期小鼠子宫内膜持续表达VEGF,且在植入前后表达最强,提示VEGF可能在小鼠胚胎的植入过程中发挥重要作用。Objective To study the expression and distribution of vascular endothelial growth factor（VEGF）and its rule in the peri-implantation mouse endometrium.Methods Immunohistochemical SP staining method was used to detected the expression of VEGF in mouse endometrium on day 3-6 of pregnancy and the unpregnant mouse endometrium（estrous stage）were selected as controls.Results Immunoreactive VEGF was detected throughout the mouse endometrial tissue,and the expression was in time-specific and cell-specific.During the pregnancy of day 3,the expression was detected weakly in luminal epithelium and endometrial stromal cells.Day 4-5,VEGF in stromal cells and luminal epithelium expressed the strongest.On day 6,VEGF expression was stronger,but mainly expressed in decidua cells.Conclusion VEGF express continuously in mouse endometrium during day 3-6 of pregnancy,and the strongest expression is before and after the implantation.It suggests that VEGF might play an key role in the process of the mouse embryo implantation.

关 键 词：血管内皮生长因子类 胚胎植入 子宫内膜 小鼠 

分 类 号：R321.3[医药卫生—人体解剖和组织胚胎学] R322.65[医药卫生—基础医学]