子宫内膜样腺癌中Id-1、Smad4和p21^(WAF1/CIP1)蛋白及mRNA的表达  

Expressions of Id-1,Smad4 and p21^(WAF1/CIP1) protein and mRNA in endometrial adenocarcinomas and their significance

作　　者：陈晶晶[1] 陈柯[1] 王晓秋[1] 胡闻[1] 

机构地区：[1]安徽医科大学附属省立医院病理科,合肥230001

出　　处：《安徽医科大学学报》2010年第1期39-43,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省教育厅自然科学研究项目(编号:2006KJ377B)

摘　　要：目的探讨分化抑制因子-1(Id-1)、DPC4/Smad4和p21WAF1/CIP1蛋白及其mRNA在正常子宫内膜、子宫内膜增殖症和子宫内膜样腺癌(EA)组织中的表达及其临床病理学意义。方法采用免疫组织化学和原位分子杂交方法,检测30例正常子宫内膜组织、30例单纯性增生过长、30例复杂性增生伴不典型增生、72例EA组织中Id-1、Smad4和p21WAF1/CIP1蛋白及其mRNA的表达。结果Id-1、Smad4和p21WAF1/CIP1蛋白及三者mRNA在EA中的表达率分别为86.1%(62/72)、44.4%(32/72)、63.9%(46/72)、73.6%(53/72)、41.7%(30/72)和61.1%(44/72),Id-1和p21WAF1/CIP1阳性率明显高于正常和各型增生内膜;而Smad4则相反;Id-1蛋白及mRNA过表达与组织学分级、临床分期、浸润程度和淋巴结转移有关(P值均<0.05);Smad4和p21WAF1/CIP1蛋白及mRNA阳性表达与组织学分级、临床分期和浸润程度有关(P值均<0.05);Id-1、Smad4和p21WAF1/CIP1蛋白与相对应的mRNA呈正相关;Id-1与Smad4表达呈负相关。结论Id-1、Smad4和p21WAF1/CIP1蛋白及mRNA表达异常与EA的发生发展有关,有可能成为判断子宫内膜组织癌变和转移的重要指标。Objective To explore the relationship of the expression of Id-1,Smad4 and p21^WAF1/CIP1 protein and mRNA in the development of endometrioid adenocarcinoma.Methods The expression levels of Id-1,Smad4 and p21^WAF1/CIP1 proteins and mRNA in endometrioid adenocarcinoma tissues（n=72）,endometrial hyperplasia tissues（n=60）and normal tissues（n=30）were examined by immunohistochemistry and in situ hybridization method.Results The positive rates of Id-1,Smad4 and p21^WAF1/CIP1 protein and mRNA in normal endometrium,endometrial hyperplasia,endometrial atypical hyperplasia and endometrioid adenocarcinoma were respectively 86.1%（62/72）、44.4%（32/72）、63.9%（46/72）、73.6%（53/72）、41.7%（30/72）and 61.1%（44/72）.The positive rates of Id-1 and p21^WAF1/CIP1 protein and mRNA were high than that in normal endometrium and endometrial hyperplasia,the opposite of Smad4.The positive expression of Id-1 was related to the histological grade（P〈0.01）,FIGO stage（P〈0.01）,depth of invasion（P〈0.05）and lymph node metastasis（P〈0.05）.The positive expression of Smad4 and p21^WAF1/CIP1 was related to the histological grade（P〈0.05）,FIGO stage（P〈0.05）and depth of invasion（P〈0.05）.The expression of Id-1 protein was positively correlated with its mRNA.The expression of Id-1 was negatively correlated with Smad4.Conclusion The expression of Id-1,Smad4 and p21^WAF1/CIP1 protein and mRNA associate with the development and progression of endometrioid adenocarcinoma,which may be a important biomarker to diagnose the early carcinogenesis of endometrioid adenocarcinoma.

关 键 词：子宫内膜样腺癌 ID-1 SMAD4 P21WAF1/CIP1 

分 类 号：R737.33[医药卫生—肿瘤]