慢性饮酒对大鼠左心室心肌胶原含量及舒张功能的影响  

Effect of Chronic Alcohol Consumption on Left Ventricular Myocardial Collagen Content and Diastolic Function in Rats

作　　者：林美光[1] 王佩显[1] 崔让庄[2] 曹丽[1] 王伟强[1] 张丽[1] 陈倩[2] 赵福梅[2] 

机构地区：[1]天津医科大学总医院心血管内科,300052 [2]天津心血管病研究所

出　　处：《天津医药》2010年第2期131-133,共3页Tianjin Medical Journal

摘　　要：目的:检测慢性饮酒大鼠左心室心肌胶原含量的变化和舒张功能的损害,并探讨两者的相关关系。方法:雄性8周龄Wistar大鼠24只,随机分为乙醇组和对照组,每组12只。喂养16周后,超声心动图和组织多普勒成像(tissue Doppler imaging,TDI)检测左心室舒张功能,羟脯氨酸测试试剂盒测定心肌羟脯氨酸含量,RT-PCR方法检测心肌Ⅰ型、Ⅲ型胶原mRNA的表达。结果:乙醇组与对照组比较,多普勒二尖瓣E峰峰值降低(P<0.01)、等容舒张时间(IVRT)延长(P<0.01);TDI二尖瓣内环舒张早期峰速度Ea降低(P<0.05),舒张晚期峰速度Aa增高(P<0.01),乙醇组的Ea/Aa比值<1,而对照组>1(P<0.01)。乙醇组的羟脯氨酸含量、Ⅰ型胶原和Ⅲ型胶原的mRNA表达水平及Ⅰ型/Ⅲ型胶原比值都高于对照组(P<0.01)。心肌羟脯氨酸含量、Ⅰ型胶原和Ⅲ型胶原的mRNA表达水平及Ⅰ型/Ⅲ型胶原比值都与IVRT呈正相关(P<0.05),与Ea/Aa比值呈负相关(P<0.01)。结论:乙醇喂养大鼠16周后左心室心肌胶原合成增加,舒张功能减低,二者呈正相关。Objective：To investigate the effect of chronic alcohol consumption on both left ventricular myocardial collagen and diastolic function in rats,and their relationship thereof.Methods：Twenty-four male Wistar rats were randomly divided into 2 groups：control group（n=12） and ethanol group（n=12）.The changes in cardiac diastolic function were evaluated by echocardiography and tissue Doppler imaging （TDI）.The value of myocardial hydroxyproline content was determined by hydroxyproline reagent kit.The expressions of collagenⅠand collagen Ⅲ mRNA were detected by RT-PCR analysis.Results：It was found that mitral E and mitral annulus Ea were decreased,mitral annulus Aa was increased,and isovolumic relaxation time （IVRT） was prolonged in the ethanol group compared with those in control group（P〈0.05）.The value of Ea/Aa ratio was greater than 1 in control group and less than 1 in ethanol group （P〈0.01）.It was found that myocardial hydroxyproline content,collagenⅠ,collagen Ⅲ mRNA expression and their ratio significantly increased in ethanol group compared with those in the control group （P〈0.01）.There was positive correlation between hydroxyproline content,collagenⅠ,collagen Ⅲ mRNA expression,and collagenⅠ/collagen Ⅲ mRNA ratio with IVRT（P〈0.05）,and negative correlation between hydroxyproline content,collagenⅠ,collagen Ⅲ mRNA expression,and collagenⅠ/collagen Ⅲ mRNA ratio with the Ea/Aa ratio （P〈0.01）.Conclusion：Chronic ethanol consumption can induce increase in left ventricular myocardial collagen synthesis and impairment in diastolic function in rats.Left ventricular diastolic dysfunction correlates with increase in myocardial collagen synthesis positively.

关 键 词：乙醇 大鼠 WISTAR 心肌 胶原 超声心动描记术 

分 类 号：R-332[医药卫生]