结核分枝杆菌耐利福平rpoB基因突变的序列分析  

Sequencing analysis on rpoB gene mutation of rifampin-resistance in Mycobacterium tuberculosis

作　　者：甘辛[1] 陈玲[1] 王建华[1] 李娜娜[1] 陈杨[1] 张建勇[1] 张泓[1] 

机构地区：[1]遵义医学院附属医院呼吸内科,贵州遵义563003

出　　处：《基础医学与临床》2010年第3期268-271,共4页Basic and Clinical Medicine

基　　金：贵州省科学技术基金(黔科合J字[2007]2223号);美国疾病控制与预防中心(美国CDC)资助项目(CDCSBIR Award#200-2007-M-22792)

摘　　要：目的探讨结核分枝杆菌耐药基因rpoB突变与利福平耐药性的关系。方法采集81例临床标本,分离结核分枝杆菌菌株,PCR扩增rpoB基因及测序,并与利福平药物敏感试验结果比较。结果27例耐利福平菌株中,20株发生rpoB基因突变(74.1%),突变位点包括531和526在内的7个位点10种突变类型,并发现新的突变位点和突变形式;54株敏感株中,1株发生突变(1.9%)。结论利福平耐药rpoB基因突变有一定区域性,为发展快速的基因诊断技术检测耐多药结核病奠定了基础。Objective Genetic evaluation of relationship between rpoB gene mutation and rifampin-resistance of Mycobacterium tuberculosis（MTB）.Methods This study was carried out with 81 clinical isolates and sensitivity test and genotypic analysis by PCR amplification and sequencing of rpoB gene were performed.Results Among these isolates,there were 27 rifampin-resistant and 54 sensitive strains.Of the 27 drug-resistant isolates,20 （74.1%） carried mutations on the amplified fragment of the rpoB gene with 10 mutation types at seven codons including 531 and 526,and two new mutation patterns were recognized.On the other hand,one mutation （1.9%） appeared in 54 drug-sensitive strains.Conclusion The study shows geographical variation in the mutation types of rpoB gene in M.tuberculosis isolates from Guizhou,China.The result is valuable for the development of fast diagnostic methods.

关 键 词：结核分枝杆菌 利福平 RPOB基因 突变 药物敏感试验 

分 类 号：R450[医药卫生—治疗学]