血管平滑肌细胞中ERK通路与TGF-β1/Smad通路的相互作用  

Interaction between TGF-β_1/Smad pathway and ERK pathway in vascular smooth muscle cells

作　　者：钟华[1,2] 何芳[1,4] 胡清华[1,4] 王振焕[1] 邓峰美[1,2] 孙志萍 李增春 

机构地区：[1]新疆地方病与民族高发病教育部重点实验室 [2]石河子大学医学院病理生理教研室 [3]石河子大学医学院医学机能实验中心,石河子832002 [4]华中科技大学同济医学院病理生理学系,卫生部呼吸系疾病重点实验室,武汉430030

出　　处：《中国应用生理学杂志》2010年第1期15-18,共4页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助(30760077)

摘　　要：目的:探讨血管平滑肌细胞(VSMC)中TGF-β/Smad与ERK信号转导通路是否存在相互调节关系。方法:原代培养的大鼠胸主动脉平滑肌细胞,分四组:①对照组,②TGF-β1组,③ERK阻断剂(PD98059)组和④TGF-β1+ERK阻断剂(PD98059)组。分别用Western blot法检测VSMC内Smad2/3、ERK1/2蛋白表达及磷酸化Smad2/3、磷酸化ERK1/2蛋白含量,RT-PCR方法测VSMC中Smad2、Smad3mRNA的表达。结果:①与对照组相比,TGF-β1组P-Smad2/3、P-ERK1/2蛋白含量增多(P<0.05),ERK阻断剂组P-Smad2/3、P-ERK1/2蛋白含量减少(P<0.05),TGF-β1+ERK阻断剂组P-Smad2/3、P-ERK1/2蛋白含量无差异;与TGF-β1组相比,TGF-β1+ERK阻断剂组P-Smad2/3、P-ERK1/2蛋白含量减少(P<0.05)。各组间Smad2/3、ERK1/2蛋白表达无差异。②各组的Smad2、Smad3mRNA表达无差异。结论:TGF-β1诱导的Smad2/3蛋白磷酸化依赖ERK通路激活,但ERK通路对Smad2/3蛋白和mRNA表达水平无影响。Objective：To investigate if the interaction between TGF-β1/Smad pathway and ERK pathway in vascular smooth muscle cells exists.Methods：The rat arota was removed.The primary VSMC were isolated and cultured in vitro,then the VSMC were divided into four groups：①control group,②TGF-β1 group,③ERK blocking agent group,④TGF-β1＋ ERK blocking agent group.The expression of Smad2/3,ERK1/2 proteins,the content of phosphorylated ERK1/2 and Smad2/3 proteins were detected by Western blot,and the expression of Smad2/3 mRNA was detected by reverse transcription-polymerase chain reaction（RT-PCR）.Results：①In contrast to control group,the content of phosphorylated Smad2/3 and phosphorylated ERK1/2 proteins in TGF-β1 group was increased（P〈0.05）,that in ERK blocking agent group was decreased（P〈0.05）.There was no difference between control group and TGF-β1＋ERK blocking agent group.Compared with TGF-β1 group,the contents of phosphorylated Smad2/3 and phosphorylated ERK1/2 proteins in TGF-β1＋ERK blocking agent group was decreased（P〈0.05）.There was no difference in the expression of Smad2/3 and ERK1/2 proteins among different groups.②There were no differences in expression of Smad2 and Smad3 mRNA among different groups.Conclusion：①TGF-β1 can induce Smad2/3 proteins to be phosphorylated dependent on the activated ERK pathway.②ERK pathway does not effect the expression of Smad2/3 at the level of protein and mRNA.

关 键 词：ERK 血管平滑肌细胞 TGF-β_1 

分 类 号：R363.2[医药卫生—病理学]